Medium for stem cell use

• US 10,457,911 B2
• Filed: 09/26/2016
• Issued: 10/29/2019
• Est. Priority Date: 03/31/2014
• Status: Active Grant

• Current Assignee: Ajinomoto Co., Inc.
• Original Assignee: Ajinomoto Co., Inc.
• Inventors: Kuriyama, Yoko, Okamoto, Satoru, Kitazawa, Manabu, Sugimoto, Nao, Matsumoto, Takuya
• Primary Examiner(s): Noble, Marcia S
• Application Number:
  US15/275,586
• Publication Number:
  US 20170009200A1
• Time in Patent Office: 1,128 Days
• CPC Class Codes: C12N 2500/50 Soluble polymers, e.g. polyethyleneglycol [PEG]
• CPC Class Codes: C12N 2501/998 Proteins not provided for elsewhere
• CPC Class Codes: C12N 5/0018 Culture media for cell or tissue culture media for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0696 Artificially induced pluripotent stem cells, e.g. iPS

Abstract

Culture media which contain a water-soluble polymer, such as polyvinyl alcohol and the like, and albumin, and the like, are useful for the proliferation of stem cells, and show good culture results, particularly with iPS cells, and maintain the stability of the medium properties, as well as using a reduced amount of albumin.

Claims

1. A medium for culturing an iPS cell, comprising:

polyvinyl alcohol; and

an albumin,

wherein the albumin and the polyvinyl alcohol are included in the medium in a weight ratio of 1;

1.1 to 100, and

an amount of fatty acid binding to the albumin is not more than 2.2 mg per 1 g of the albumin.

2. The medium according to claim 1, wherein the polyvinyl alcohol has a critical micelle concentration of 0.1 to 5 mg/ml.

3. The medium according to claim 1, wherein the polyvinyl alcohol has a hydrolysis ratio of 60 to 95%.

4. The medium according to claim 2, wherein the polyvinyl alcohol has a hydrolysis ratio of 60 to 95%.

6. A method of culturing an iPS cell, comprising:

culturing the iPS cell in the culture medium according to claim 1.

7. A method of culturing an iPS cell, comprising:

culturing the iPS cell in the culture medium according to claim 2.

8. A method of culturing an iPS cell, comprising:

culturing the iPS cell in the culture medium according to claim 3.

9. A cell culture, comprising an iPS cell in the culture medium according to claim 1.

10. The medium according to claim 1, wherein the albumin comprises recombinant human albumin.

11. The medium according to claim 1, wherein the albumin and the polyvinyl alcohol are included in the medium in the weight ratio of 1:

1.1 to 15.

14. The medium according to claim 1, wherein the amount of fatty acid binding to the albumin is from 1 to 2 mg per 1 g of the albumin.

5. A method of preventing degradation of an albumin-containing medium, comprising:

adding polyvinyl alcohol to the medium,

wherein a weight ratio of the albumin in the medium to the polyvinyl alcohol added to the medium is 1;

1.1 to 100, and

an amount of fatty acid binding to the albumin is not more than 2.2 mg per 1 g of the albumin.

12. The method according to claim 5, wherein the albumin in the medium comprises recombinant human albumin.

13. The method according to claim 5, wherein the weight ratio of the albumin in the medium to the polyvinyl alcohol added to the medium is 1:

1.1 to 15.

15. The method according to claim 5, wherein the amount of fatty acid binding to the albumin is from 1 to 2 mg per 1 g of the albumin.