Methods for diagnosing infectious diseases using adsorption media

• US 10,457,974 B2
• Filed: 11/02/2016
• Issued: 10/29/2019
• Est. Priority Date: 11/08/2013
• Status: Active Grant

• Current Assignee: ExThera Medical Corporation
• Original Assignee: ExThera Medical Corporation
• Inventors: Ward, Robert S., McCrea, Keith R.
• Primary Examiner(s): Hines, Jana A
• Application Number:
  US15/341,416
• Publication Number:
  US 20170073727A1
• Time in Patent Office: 1,091 Days
• Field of Search: 435 5, 435 612, 435 792, 435 34, 4352872, 422527
• CPC Class Codes: B01D 15/3828 Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
• CPC Class Codes: C12Q 1/24 Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 1/70 involving virus or bacteriophage immunoassay for viruses G01N33/56983
• CPC Class Codes: C12Q 1/701 Specific hybridization probes
• CPC Class Codes: C12Q 1/707 non-A, non-B Hepatitis, excluding hepatitis D
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G01N 2333/005 from viruses
• CPC Class Codes: G01N 2333/195 from bacteria
• CPC Class Codes: G01N 2333/43556 from ticks
• CPC Class Codes: G01N 2333/44 from protozoa
• CPC Class Codes: G01N 2333/445 Plasmodium
• CPC Class Codes: G01N 2469/10 Detection of antigens from microorganism in sample from host
• CPC Class Codes: G01N 33/569 for microorganisms, e.g. protozoa, bacteria, viruses
• CPC Class Codes: G01N 33/56905 Protozoa
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention provides an in vitro method for concentrating infectious pathogens found in a biological sample obtained from an individual who is suspected of being infected with the pathogens. Provided herein is also an in vitro method for reducing or eliminating blood cells from a sample obtained from an individual suspected to being infected with an infectious pathogen. The present invention also provides a method for diagnosing malaria and a method for determining if an individual is infected with a pathogen. Provided herein is also a concentrator and a kit for use with the methods.

Claims

1. An in vitro method for removing a bacteria of genus Borrelia from a subject, the method comprising:

(a) contacting a blood sample obtained from said subject with an adsorption media under conditions to form an adhering complex comprising the adsorption media and a cell present in the blood sample which is infected with the bacteria; and

(b) removing the adhering complex from the blood sample, to thereby remove the bacteria of genus Borrelia and treat Lyme disease.

2. The method of claim 1, wherein the bacteria is selected from a group consisting of:

Borrelia burgdorferi, Borrelia mayonii, Borrelia afzelii, Borrelia garinii, and any combination thereof.

3. The method of claim 1, wherein the adhering complex further comprises a cellular byproduct of the bacteria responsible for causing an immune response syndrome associated with Jarisch-Herxheimer reaction in the subject.

4. The method of claim 1, wherein the adsorption media is a solid substrate of high surface area having at least one polysaccharide adsorbent on the surface thereof.

5. The method of claim 4, wherein the at least one polysaccharide adsorbent is attached to the surface of the solid substrate by end-point attachment.

6. The method of claim 4, wherein said solid substrate comprises a plurality of rigid polymer beads.

7. The method of claim 6, wherein said plurality of rigid polymer beads are rigid polyethylene beads.

8. The method of claim 7, wherein the at least one polysaccharide adsorbent is a member selected from the group consisting of heparin, heparan sulfate, mannose, dextran sulfate, hyaluronic acid, salicylic acid, chitosan, polyethylenimine (PEI) and a combination thereof.

9. The method of claim 8, wherein the at least one polysaccharide adsorbent is heparin.

10. The method of claim 9, wherein the at least one polysaccharide adsorbent further comprises chitosan, PEI or a combination.

11. The method of claim 1, wherein prior to step (a), a blood sample is taken from the subject to determine the presence Borrelia burgdorferi using heparinized beads.