Skin topography as a marker for skin maturation

• US 10,458,977 B2
• Filed: 05/15/2015
• Issued: 10/29/2019
• Est. Priority Date: 05/15/2014
• Status: Active Grant

• Current Assignee: Laboratoires Expanscience
• Original Assignee: Laboratoires Expanscience
• Inventors: Lachman, Nadege, Msika, Philippe, Fluhr, Joachim
• Primary Examiner(s): Shah, Utpal D
• Application Number:
  US15/310,303
• Publication Number:
  US 20170241985A1
• Time in Patent Office: 1,628 Days
• CPC Class Codes: A61B 5/442 Evaluating skin mechanical properties, e.g. elasticity, hardness, texture, wrinkle assessment
• CPC Class Codes: A61B 5/4848 Monitoring or testing the effects of treatment, e.g. of medication
• CPC Class Codes: G01N 33/5026 on cell morphology
• CPC Class Codes: G01N 33/5044 involving specific cell types
• CPC Class Codes: G06T 2207/10056 Microscopic image
• CPC Class Codes: G06T 2207/30088 Skin; Dermal
• CPC Class Codes: G06T 7/0012 Biomedical image inspection

Abstract

The present invention relates to a method for determining the relief of the skin surface of a subject, and hence the degree of maturation of the said skin surface. Methods for identifying active agents, raw materials and formulation are also provided.

Claims

1. A method, comprising:

a) obtaining at least one sample (A) of skin cells of a child;

b) contacting a candidate cosmetic product with the sample (A);

c) obtaining an image of the sample (A) after step b) using methods of optical microscopy, scanning probe microscopy, electronic microscopy, optical tomography or optical profiling;

d) measuring the developed surface of the skin of the sample (A);

e) obtaining at least one control sample of skin wherein said sample has not been in contact with the candidate cosmetic product of step b);

f) obtaining an image of the control sample of step e) using methods of optical microscopy, scanning probe microscopy, electronic microscopy, optical tomography or optical profiling;

g) measuring the developed surface of the skin of said control sample;

h) preparing a dermo-cosmetic composition for the skin of a child comprising the candidate cosmetic product of step b) when the developed surface measured in step d) is superior or equal to the developed surface measured in step g).

2. The method of claim 1, further comprising the step of determining the body surface area (BSA) of the child of step a).

3. The method claim 2, further comprising the steps of determining the ratio between the BSA of said child and the average BSA value in the adult population, and determining the corrected developed surface of the skin sample (A) of step a) by multiplying the developed skin surface of step d) by said ratio.

4. The method of claim 1, wherein said skin sample (A) is a replica of the skin surface of the child of step a).

5. The method of claim 1, wherein:

said methods of optical microscopy are selected from the group consisting of conventional light microscopy, fluorescence microscopy, confocal/multiphoton microscopy and stimulated emission depletion;

said methods of scanning probe microscopy are selected from the group consisting of scanning tunneling microscopy (STM), atomic force microscopy (AFM), near-field scanning optical microscopy; and

said method of optical tomography is optical coherence tomography.

6. The method of claim 1, wherein the image of step c) and the measurement of the developed skin surface of step d) for said sample (A) is obtained by using optical profiling.

7. The method of claim 6, wherein optical profiling includes confocal microscopy and low coherence interferometry.

8. The method of claim 1, wherein the child of step a) is a newborn of 1 to 15 days, an infant of 5/6 weeks, an infant of 6 months±

1 month, an infant of 1 year an infant of 4 to 5 years, 7 years or 10 years.

9. The method of claim 1, further comprising the step i) of administering an effective amount of the dermo-cosmetic composition of step h) to the skin of the child of step a).

10. A method, comprising:

a) obtaining at least one sample (A′

) of skin cells of a child affected by a skin disorder;

b) obtaining an image of the sample (A′

) of step a) using methods of optical microscopy, scanning probe microscopy, electronic microscopy, optical tomography or optical profiling;

c) measuring the developed surface of the skin of said sample (A′

);

d) obtaining at least one control sample (B) of skin cells of a healthy child;

e) obtaining an image of the sample (B) of step d) using methods of optical microscopy, scanning probe microscopy, electronic microscopy, optical tomography or optical profiling;

f) measuring the developed surface of the skin of said sample (B);

g) administering a composition or the formulation to the skin of the child of step a) for treating said skin disorder when the developed surface measured in step f) is superior or equal to the developed surface measured in step c).

11. The method of claim 10, further comprising the step of determining the body surface area (BSA) of the child of step a).

12. The method claim 11, further comprising the steps of determining the ratio between the BSA of said child and the average BSA value in the adult population, and determining the corrected developed surface of the skin sample (A′

) of step a) by multiplying the developed skin surface of step c) by said ratio.

13. The method of claim 10, wherein said skin sample (A′

) is a replica of the skin surface of the child of step a).

14. The method of claim 10, wherein:

said methods of optical microscopy are selected from the group consisting of conventional light microscopy, fluorescence microscopy, confocal/multiphoton microscopy and stimulated emission depletion;

said methods of scanning probe microscopy are selected from the group consisting of scanning tunneling microscopy (STM), atomic force microscopy (AFM), near-field scanning optical microscopy; and

said method of optical tomography is optical coherence tomography.

15. The method of claim 10, wherein the image of step b) and the measurement of the developed skin surface of step c) for said sample (A′

) is obtained by using optical profiling.

16. The method of claim 15, wherein optical profiling includes confocal microscopy and low coherence interferometry.

17. The method of claim 10, wherein the child of step a) is a newborn of 1 to 15 days, an infant of 5/6 weeks, an infant of 6 months±

1 month, an infant of 1 year an infant of 4 to 5 years, 7 years or 10 years.

18. A method, comprising:

a) obtaining at least one sample (A′

) of skin cells of a child affected by a skin disorder;

b) contacting a candidate active agent with the sample (A′

) of step a);

c) obtaining an image of the sample (A′

) of step b) using methods of optical microscopy, scanning probe microscopy, electronic microscopy, optical tomography or optical profiling;

d) measuring the developed surface of the skin of said sample (A′

);

e) obtaining at least one control sample of skin cells wherein said sample has not been in contact with the candidate active agent of step b);

f) obtaining an image of the control sample of step e) using methods of optical microscopy, scanning probe microscopy, electronic microscopy, optical tomography or optical profiling;

g) measuring the developed surface of the skin of said control sample;

h) preparing a pharmaceutical formulation for children comprising the candidate active agent of step b) when the developed surface measured in step d) is superior or equal to the developed surface measured in step g).

19. The method of claim 18, further comprising the step of determining the body surface area (BSA) of the child of step a).

20. The method claim 19, further comprising the steps of determining the ratio between the BSA of said child and the average BSA value in the adult population, and determining the corrected developed surface of the skin sample (A′

) of step a) by multiplying the developed skin surface of step d) by said ratio.

21. The method of claim 18, wherein said skin sample (A′

) is a replica of the skin surface of the child of step a).

22. The method of claim 18, wherein:

said methods of optical microscopy are selected from the group consisting of conventional light microscopy, fluorescence microscopy, confocal/multiphoton microscopy and stimulated emission depletion;

said methods of scanning probe microscopy are selected from the group consisting of scanning tunneling microscopy (STM), atomic force microscopy (AFM), near-field scanning optical microscopy; and

said method of optical tomography is optical coherence tomography.

23. The method of claim 18, wherein the image of step c) and the measurement of the developed skin surface of step d) for said sample (A′

) is obtained by using optical profiling.

24. The method of claim 23, wherein optical profiling includes confocal microscopy and low coherence interferometry.

25. The method of claim 18, wherein the child of step a) is a newborn of 1 to 15 days, an infant of 5/6 weeks, an infant of 6 months±

1 month, an infant of 1 year an infant of 4 to 5 years, 7 years or 10 years.

26. The method of claim 18, further comprising the step i) of administering an effective amount of the pharmaceutical formulation of step h) to the skin of the child of step a).