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Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids

  • US 10,458,978 B2
  • Filed: 02/13/2017
  • Issued: 10/29/2019
  • Est. Priority Date: 08/22/2006
  • Status: Active Grant
First Claim
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1. A device for detecting the presence of a target nucleic acid in a fluid sample, the device comprising:

  • a porous lateral flow strip having a width of 3 mm or less; and

    a microarray of spots on said porous lateral flow strip, each spot comprising a plurality of capture oligonucleotides;

    wherein each capture oligonucleotide is complementary to a capture sequence of the target nucleic acid;

    wherein each spot was printed with a solution having a concentration of capture oligonucleotides between 200 μ

    M and 800 μ

    M; and

    wherein said microarray of spots provides a quantitative visual colorimetric signal indicating an amount of from 250 amol to 1 fmol of the target nucleic acid in the fluid sample comprising at least 150 mM salt in less than two minutes by capillary flow.

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