Three-step acid dissociation enzyme linked immunosorbent (TADELIS) assay
First Claim
1. A method for determining in a sample the total amount of a ligand of a ligand-binding protein therapeutic comprising the following steps in the following order:
- subjecting the sample to an acid treatment,forming in solution a ternary complex comprisingi) an anti-ligand antibody,ii) the ligand, andiii) labelled ligand-binding protein,by adding first the labelled ligand-binding protein to the sample to form a binary labelled ligand-binding protein-ligand complex, and by adding after the formation of the binary complex the anti-ligand antibody to the sample to form a ternary labelled ligand-binding protein-ligand-anti-ligand antibody complex, anddetermining the amount of the ternary complex,andthereby determining the amount of the ligand of the ligand-binding protein.
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Abstract
Herein is reported a method for determining in a sample the (total, i.e. binding competent) amount of a ligand of a ligand-binding protein (therapeutic) comprising the following steps in the following order: subjecting the sample to an acid treatment, forming in solution a non-covalent complex comprising i) an anti-ligand antibody, ii) the ligand, and iii) labelled ligand-binding protein, by adding anti-ligand antibody and labelled ligand-binding protein to the sample, and determining the amount of the complex and thereby determining the amount of the ligand of the ligand-binding protein.
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Citations
17 Claims
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1. A method for determining in a sample the total amount of a ligand of a ligand-binding protein therapeutic comprising the following steps in the following order:
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subjecting the sample to an acid treatment, forming in solution a ternary complex comprising i) an anti-ligand antibody, ii) the ligand, and iii) labelled ligand-binding protein, by adding first the labelled ligand-binding protein to the sample to form a binary labelled ligand-binding protein-ligand complex, and by adding after the formation of the binary complex the anti-ligand antibody to the sample to form a ternary labelled ligand-binding protein-ligand-anti-ligand antibody complex, and determining the amount of the ternary complex, and thereby determining the amount of the ligand of the ligand-binding protein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification