Anterior endoderm cells and methods of production
First Claim
Patent Images
1. An in vitro method for producing human anterior foregut endoderm cells, comprising:
- differentiating human pluripotent stem cells to definitive endoderm cells, andcontacting the definitive endoderm cells with an effective amount of a retinoid to produce human anterior foregut endoderm cells.
2 Assignments
0 Petitions
Accused Products
Abstract
Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed.
-
Citations
20 Claims
-
1. An in vitro method for producing human anterior foregut endoderm cells, comprising:
-
differentiating human pluripotent stem cells to definitive endoderm cells, and contacting the definitive endoderm cells with an effective amount of a retinoid to produce human anterior foregut endoderm cells. - View Dependent Claims (2, 3, 4)
-
-
5. An in vitro method for producing human midgut endoderm cells, comprising:
-
differentiating human pluripotent stem cells to definitive endoderm cells, and contacting the definitive endoderm cells with an effective amount of a retinoid to produce human midgut endoderm cells. - View Dependent Claims (6, 7)
-
-
8. An in vitro method for producing human hindgut endoderm cells, comprising:
-
differentiating human pluripotent stem cells to definitive endoderm cells, and contacting the definitive endoderm cells with an effective amount of a retinoid to produce human hindgut endoderm cells. - View Dependent Claims (9, 10)
-
-
11. An in vitro method for producing anterior foregut endoderm cells, midgut endoderm cells and/or hindgut endoderm cells, comprising:
-
differentiating human pluripotent stem cells to definitive endoderm cells, and contacting the definitive endoderm cell population with a retinoid in an amount sufficient to produce the human anterior foregut endoderm cells, midgut endoderm cells and/or human hindgut endoderm cells. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
-
Specification