Detection of nucleic acids from multiple types of human papillomavirus
First Claim
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1. A combination of oligomers for detecting human papillomavirus (HPV) types in group D, the combination comprising:
- a first amplification oligomer comprising a target-specific sequence and a 5′
promoter sequence, wherein the first amplification oligomer target-specific sequence consists of SEQ ID NO;
35 or SEQ ID NO;
37, or an RNA equivalent thereof; and
a second amplification oligomer comprising a target-specific sequence consisting of SEQ ID NO;
41, or an RNA equivalent thereof.
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Abstract
Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.
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Citations
20 Claims
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1. A combination of oligomers for detecting human papillomavirus (HPV) types in group D, the combination comprising:
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a first amplification oligomer comprising a target-specific sequence and a 5′
promoter sequence, wherein the first amplification oligomer target-specific sequence consists of SEQ ID NO;
35 or SEQ ID NO;
37, or an RNA equivalent thereof; anda second amplification oligomer comprising a target-specific sequence consisting of SEQ ID NO;
41, or an RNA equivalent thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for of determining the presence or absence of a human papillomavirus (HPV) nucleic acid type in group D in a sample, comprising the steps of:
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(a) contacting a sample, said sample suspected of containing a target nucleic acid of an HPV type in group D, with a combination of amplification oligomers that amplify a target region of said target nucleic acid, wherein (i) a first amplification oligomer comprises a target-specific sequence and a 5′
promoter sequence, wherein the first amplification oligomer target-specific sequence consists of SEQ ID NO;
35 or SEQ ID NO;
37, or an RNA equivalent thereof; and(ii) a second amplification oligomer comprises a target-specific sequence consisting of SEQ ID NO;
41, or an RNA equivalent thereof;(b) performing an amplification reaction using the amplification oligomers, wherein if the target nucleic acid of the HPV type in group D is present in the sample, an amplification product is produced; and (c) detecting the amplification product. - View Dependent Claims (15, 16, 17, 18, 19, 20)
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Specification