Methods and apparatuses for detecting microscope slide coverslips

• US 10,466,147 B2
• Filed: 07/23/2018
• Issued: 11/05/2019
• Est. Priority Date: 03/05/2013
• Status: Active Grant

• Current Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Original Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Inventors: Thompson, Michael J., Hassen, Charles Nathan
• Primary Examiner(s): Turk, Neil N
• Application Number:
  US16/043,110
• Publication Number:
  US 20190041301A1
• Time in Patent Office: 470 Days
• Field of Search: None
• CPC Class Codes: B65H 7/14 by photoelectric feelers or detectors
• CPC Class Codes: G01J 1/0238 making use of sensor-related data, e.g. for identification of sensor or optical parts
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00039 Transport arrangements specific to flat sample substrates, e.g. pusher blade
• CPC Class Codes: G01N 2035/00059 vacuum chucks
• CPC Class Codes: G01N 2035/00079 Evaporation covers for slides
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G02B 1/11 Anti-reflection coatings
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides

Abstract

A system is capable of detecting substrates and can differentiate between zero, one, or multiple transparent or semi-transparent substrates in a stack. The system can include an optical sensor, an optically anti-reflective element, and a detector. The optical sensor outputs light towards the optically anti-reflective element. The light detector is positioned to detect light from the light source that is reflected by substrates, if any, positioned within a detection zone between the optically anti-reflective element and the detector.

Claims

1. An automated microscope processing apparatus, comprising:

a processing station configured to robotically apply liquid to specimen-bearing microscope slides, wherein the processing station has one or more nozzles configured to robotically apply the liquid onto the specimen-bearing microscope slides; and

a coverslip detector including

a coverslip holder mechanism for holding a coverslip, wherein the coverslip holder mechanism is selected from a group consisting of a coverslip holder vacuum mechanism and a coverslip holder mechanical mechanism,

an anti-reflective optical element positioned along the coverslip holder mechanism,

a reflectance sensor including a light source configured to output light towards the anti-reflective optical element and a light detector configured to detect the outputted light, which has been reflected by any coverslips carried by the coverslip holder mechanism, and

a controller communicatively coupled to the reflectance sensor and programmed to determine whether the coverslip holder mechanism is carrying more than one coverslip based on one or more signals from the reflectance sensor indicative of the detected light.

2. The coverslip detector of claim 1, further comprising a transport mechanism configured to robotically move the coverslip holder mechanism from a coverslip pick up position to a coverslip detection position, wherein the transport mechanism includes at least one of a robotic arm, a conveyor, a rail assembly, or combinations thereof, wherein the controller is programmed to command the coverslip detector to transport individual coverslips held by the transport mechanism to the processing station.

3. A coverslip detector for detecting microscope slide coverslips, the coverslip detector comprising:

a coverslip holder mechanism for holding a microscope slide coverslip, the coverslip holder mechanism is a coverslip holder vacuum mechanism or a coverslip holder mechanical mechanism,

an anti-reflective optical element positioned along the coverslip holder mechanism;

a reflectance sensor including a light source configured to output light towards the anti-reflective optical element, the reflectance sensor including a light detector configured to detect the outputted light that has been reflected by any microscope slide coverslips carried by the coverslip holder mechanism and positioned between the reflectance sensor and the anti-reflective optical element; and

a controller communicatively coupled to the reflectance sensor and including one or more processors and non-transitory, computer readable media with instructions, executable by the one or more processors, to determine if the coverslip holder mechanism is carrying more than one microscope slide coverslip based on one or more signals from the reflectance sensor indicative of the detected light.

4. The coverslip detector of claim 3, wherein the anti-reflective optical element is positioned along an optical path along which the outputted light travels toward the anti-reflective optical element when the coverslip holder mechanism is at the coverslip detection position.

5. The coverslip detector of claim 3, wherein when the one or more processors execute the instructions, the controller compares data stored by the non-transitory, computer readable media and data from the reflectance sensor to determine if the coverslip holder mechanism is carrying more than one coverslip.

6. The coverslip detector of claim 3, wherein when the one or more processors execute the instructions, the controller correlates the detected light to at least one of

a microscope slide coverslip absence,

a presence of at least one microscope slide coverslip between the reflectance sensor and the anti-reflective optical element, or

a number of microscope slide coverslips between the reflectance sensor and the anti-reflective optical element.

7. The coverslip detector of claim 3, wherein when the one or more processors execute the instructions, the controller further determines if the coverslip holder mechanism is carrying a single microscope slide coverslip based on measured reflected light intensity and a stored reference light intensity.

8. The coverslip detector of claim 3, wherein the anti-reflective optical element absorbs outputted light that has passed through the microscope slide coverslip.

9. The coverslip detector of claim 3, wherein the anti-reflective optical element inhibits optical noise with the light from the reflectance sensor that is reflected by the one or more coverslips carried by the coverslip holder mechanism.

10. The coverslip detector of claim 3, wherein the coverslip holder mechanism is positionable to hold the microscope slide coverslip along an optical path from the reflectance sensor to the anti-reflective optical element.

11. The coverslip detector of claim 3, wherein the anti-reflective optical element includes a black surface, a black coating, and/or a black film.

12. The coverslip detector of claim 3, wherein the anti-reflective optical element absorbs incident light from the reflectance sensor such that an amount of light, if any, reflected by the coverslip holder mechanism and the anti-reflective optical element is less than an amount of light reflected by a single microscope slide coverslip.

13. The coverslip detector of claim 3, wherein the anti-reflective optical element absorbs most of the light from the reflectance sensor that strikes the optically anti-reflective optical element.

14. The coverslip detector of claim 3, wherein the anti-reflective optical element prevents reflection of at least a portion of incident light from the reflectance sensor to enable the reflectance sensor to detect a number of microscope slide coverslips carried by the coverslip holder mechanism.

26. The coverslip detector of claim 3, wherein the coverslip holder mechanism is the coverslip holder mechanical mechanism, which includes a plurality of pinchers and/or clamps.

15. A coverslip detector for detecting microscope slide coverslips, the coverslip detector comprising:

a coverslip holder mechanism for holding a microscope slide coverslip, wherein the coverslip holder mechanism is a coverslip holder vacuum mechanism or a coverslip holder mechanical mechanism;

an anti-reflective optical element positioned along the coverslip holder mechanism;

a photoelectric sensor that outputs light toward the anti-reflective optical element, the photoelectric sensor is positioned to detect the outputted light that has been reflected by each microscope slide coverslip carried by the coverslip holder mechanism; and

a controller communicatively coupled to the photoelectric sensor and including one or more processors and non-transitory, computer readable memory with programming for determining if the coverslip holder mechanism is holding more than one microscope slide coverslip based on the detection of the reflected light.

16. The coverslip detector of claim 15, wherein the controller determines if the coverslip holder mechanism is carrying multiple microscope slide coverslips based on the detection of the reflected light.

17. The coverslip detector of claim 15, wherein the detection of the reflected light includes measuring reflected light intensity.

18. The coverslip detector of claim 15, wherein the anti-reflective optical element has a reflectivity less than about 0.1 for the one or more wavelengths emitted by the reflectance sensor.

19. The coverslip detector of claim 15, wherein the optically anti-reflective element includes a light-absorbing, low-remission surface that is less optically reflective than glass.

20. The coverslip detector of claim 15, wherein the reflectance sensor is configured to detect the light reflected by one or more coverslips positioned within a coverslip detection zone while light from the reflectance sensor, which is transmitted through the one or more coverslips, strikes the anti-reflective optical element.

21. The coverslip detector of claim 20, further comprising a coverslip transport apparatus configured to move the coverslip holder mechanism into and out of the coverslip detection zone, wherein the coverslip transport apparatus includes at least one of a robotic arm, a conveyor, a rail assembly, a motor, or combinations thereof.

22. The coverslip detector of claim 15, wherein the reflectance sensor is spaced apart from the coverslip holder mechanism when the coverslip holder mechanism is at a coverslip detection position such that the coverslip holder mechanism is capable of holding two or more coverslips spaced apart from the reflectance sensor.

23. The coverslip detector of claim 15, wherein the anti-reflective optical element is a low-remission surface of the coverslip holder mechanism.

24. The coverslip detector of claim 15, wherein the controller is in communication with a transport apparatus operable to move the coverslip holder mechanism to sequentially retrieve microscope slide coverslips and place the retrieved microscope slide coverslip on respective microscope slides.

25. The coverslip detector of claim 15, wherein the controller is programmed to determine a number of the coverslips in a coverslip detection zone based, at least in part, on the detected light.