Optical flow cell assembly incorporating a replaceable transparent flow cell

• US 10,466,173 B2
• Filed: 10/06/2017
• Issued: 11/05/2019
• Est. Priority Date: 10/06/2017
• Status: Active Grant

• Current Assignee: Wyatt Technology Corporation
• Original Assignee: Wyatt Technology Corporation
• Inventors: Rahmlow, David, Greenstreet, Matthew, Sen, Shiladitya
• Primary Examiner(s): Amara, Mohamed K
• Application Number:
  US15/727,398
• Publication Number:
  US 20190107487A1
• Time in Patent Office: 760 Days
• CPC Class Codes: G01N 15/0211 Investigating a scatter or diffraction pattern
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 2015/1452 Adjustment of focus; Alignment
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 2021/4711 Multiangle measurement
• CPC Class Codes: G01N 2021/513 Cuvettes for scattering measurements
• CPC Class Codes: G01N 2030/746 detecting along the line of flow, e.g. axial
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/39 using tunable lasers
• CPC Class Codes: G01N 21/53 within a flowing fluid, e.g. smoke
• CPC Class Codes: G01N 21/532 with measurement of scattering and transmission
• CPC Class Codes: G01N 30/74 Optical detectors measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J

Abstract

A new liquid flow cell assembly for light scattering measurements is disclosed which utilized a floating manifold system. The assembly operates with minimal stacked tolerances by aligning the cell to the windows within a manifold and independently aligning the cell to the read head directly. This configuration enables the ability to replace the flow cell or the flow cell/manifold assembly within a light scattering instrument without the need to realign the flow through elements with the light scattering illumination source while still maintaining reproducible, quality data. Some embodiments employ wide bore cells which enable the measurement of process analytic technology (PAT) including online monitoring of reactions.

Claims

1. An optical flow cell assembly comprising:

a transparent flow cell;

a manifold configured to contain the flow cell;

a read head comprising

a beam generating light source aligned to emit a beam of light along an axis of the read head; and

wherein the read head is configured to be directly registered to the flow cell, thereby aligning the flow cell to the read head,

wherein the read head further comprises

a plurality of read head vertical alignment posts configured to contact at least one planar alignment surface of the flow cell to define a vertical read head-to-flow cell alignment plane perpendicular to the axis,

a read head top plate configured to drive the at least one planar alignment surface against the read head vertical alignment posts,

at least one read head horizontal alignment boss configured to contact an alignment step of the flow cell to define a horizontal read head-to-flow cell alignment plane perpendicular to the vertical read head-to-flow cell alignment plane, and

a ball plunger configured to drive the alignment step into contact with the at least one read head horizontal alignment boss,

wherein the plurality of read head vertical alignment posts is distinct from the read head top plate, is distinct from the at least one read head horizontal alignment boss, and is distinct from the ball plunger,

wherein the read head top plate is distinct from the at least one read head horizontal alignment boss and is distinct from the ball plunger, and

wherein the at least one read head horizontal alignment boss is distinct from the ball plunger.

2. The optical flow cell assembly of claim 1 wherein the manifold comprises:

a liquid sample inlet port;

a liquid sample outlet port;

an inlet path coupled to an outlet of the inlet port;

an outlet path coupled to the inlet of the outlet port; and

wherein the inlet path and the outlet path are configured to direct a flow of a liquid sample from the inlet port through the flow cell and out of the outlet port.

3. The optical flow cell assembly of claim 2 wherein the manifold further comprises transparent optical windows configured to allow the beam to pass into and out of the flow cell.

4. The optical flow cell assembly of claim 2 wherein the manifold further comprises a plurality of post elements incorporated into a surface of the manifold,

wherein the plurality of post elements is configured to contact the flow cell to determine a vertical registration of the flow cell relative to the manifold.

5. The optical flow cell assembly of claim 4

wherein the plurality of post elements comprises three post elements configured to define a plane upon which the flow cell rests,

wherein the at least one planar alignment surface is configured to sit upon the plane upon which the flow cell rests.

6. The optical flow cell assembly of claim 5 wherein the manifold further comprises at least one manifold horizontal alignment element configured to contact the alignment step to determine a horizontal position of the flow cell relative to the manifold.

7. The optical flow cell assembly of claim 6

wherein a horizontal distance from a flow path through the flow cell to the alignment step defines a first critical tolerance, and

wherein a vertical distance from a bore through the flow cell to the at least one planar alignment surface defines a second critical tolerance.

8. The optical flow cell assembly of claim 7 wherein the manifold further comprises vertical holes configured to allow access to the at least one planar alignment surface.

9. The optical flow cell assembly of claim 8 further comprising a compressible element configured to generate a vertical force between the manifold and the flow cell to drive the at least one planar alignment surface against the plurality of post elements.

10. The optical flow cell assembly of claim 9 wherein the compressible element comprises a wave washer configured to be placed between the flow cell and a manifold top plate configured to be rigidly connected to the manifold.

11. The optical flow cell assembly of claim 10 further comprising a spring element configured to generate a horizontal force between the manifold and the flow cell to drive the alignment step against the at least one manifold horizontal alignment element.

12. The optical flow cell assembly of claim 11 wherein the spring element comprises a leaf spring configured to be rigidly connected to the manifold and configured to be in contact with the flow cell.

13. The optical flow cell assembly of claim 12 wherein the leaf spring defines an opening along a horizontal axis of the leaf spring,

wherein the opening is configured to permit access to the flow cell.

14. The optical flow cell assembly of claim 8 further comprising a spring element configured to generate a horizontal force between the manifold and the flow cell to drive the alignment step against the at least one manifold horizontal alignment element.

15. The optical flow cell assembly of claim 4 further comprising a compressible element configured to generate a vertical force between the flow cell and the read head to drive the at least one planar alignment surface against the plurality of post elements.

16. A method comprising:

containing an optical flow cell within a manifold such that the optical flow cell is aligned to the manifold; and

registering directly the flow cell to a read head with read head-to-flow cell alignment elements, thereby aligning the flow cell to the read head,

wherein the read head comprises a beam generating light source aligned to emit a beam of light along an axis of the read head

wherein the read head further comprises

a plurality of read head vertical alignment posts configured to contact at least one planar alignment surface of the flow cell to define a vertical read head-to-flow cell alignment plane perpendicular to the axis,

a read head top plate configured to drive the at least one planar alignment surface against the read head vertical alignment posts,

at least one read head horizontal alignment boss configured to contact an alignment step of the flow cell to define a horizontal read head-to-flow cell alignment plane perpendicular to the vertical read head-to-flow cell alignment plane and

a ball plunger configured to drive the alignment step into contact with the at least one read head horizontal alignment boss,

wherein the plurality of read head vertical alignment posts is distinct from the read head top plate, is distinct from the at least one read head horizontal alignment boss, and is distinct from the ball plunger,

wherein the read head top plate is distinct from the at least one read head horizontal alignment boss and is distinct from the ball plunger, and

wherein the at least one read head horizontal alignment boss is distinct from the ball plunger.

17. The method of claim 16 further comprising

providing a vertical force between the manifold and the flow cell,

thereby allowing the vertical force to drive a vertical alignment surface of the flow cell against a plurality of vertical alignment elements incorporated into the manifold,

thereby allowing, in response to the vertical alignment surface being in contact with the plurality of vertical alignment elements, the flow cell to be in proper vertical alignment with optical windows of the manifold.

18. The method of claim 17 further comprising

providing a horizontal force between the manifold and the flow cell,

thereby allowing the horizontal force to drive a horizontal alignment surface incorporated into the flow cell against a plurality of horizontal alignment elements incorporated into the manifold,

thereby allowing, in response to the flow cell being in contact with the plurality of horizontal alignment elements, the flow cell to be in proper horizontal alignment with the windows.