Lowering blood glucose
First Claim
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1. A method for lowering blood glucose levels in a mammal comprising:
- (a) differentiating pancreatic endoderm cells in a medium comprising an exogenous retinoid to obtain a population of pancreatic endocrine precursor cells expressing insulin, NKX6.1 and PDX1, but not expressing CDX2; and
(b) transplanting the population of pancreatic endocrine precursor cells of step (a) into the liver, natural pancreas, renal subcapsular space, omentum, peritoneum, subserosal space, intestine, or a subcutaneous pocket of the mammal, wherein the pancreatic endocrine precursor cells express insulin, lower the blood glucose levels in the mammal, and are protected from host immune response when transplanted.
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Abstract
The present invention provides a method for lowering blood glucose levels in an animal by transplanting a population of pancreatic endocrine precursor cells into an animal.
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Citations
21 Claims
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1. A method for lowering blood glucose levels in a mammal comprising:
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(a) differentiating pancreatic endoderm cells in a medium comprising an exogenous retinoid to obtain a population of pancreatic endocrine precursor cells expressing insulin, NKX6.1 and PDX1, but not expressing CDX2; and (b) transplanting the population of pancreatic endocrine precursor cells of step (a) into the liver, natural pancreas, renal subcapsular space, omentum, peritoneum, subserosal space, intestine, or a subcutaneous pocket of the mammal, wherein the pancreatic endocrine precursor cells express insulin, lower the blood glucose levels in the mammal, and are protected from host immune response when transplanted. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for lowering blood glucose levels in a mammal comprising:
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(a) differentiating pancreatic endoderm cells in a medium comprising an exogenous retinoid to obtain a population of pancreatic endocrine precursor cells expressing NKX6.1 and PDX1, but not expressing CDX2; (b) differentiating the pancreatic endocrine precursor cells of step (a) in a medium to obtain a population of cells expressing insulin; and (c) transplanting the population of insulin expressing cells of step (b) into the liver, natural pancreas, renal subcapsular space, omentum, peritoneum, subserosal space, intestine, or a subcutaneous pocket of the mammal, wherein the pancreatic endocrine precursor cells express insulin, lower the blood glucose levels in the mammal, and are protected from host immune response when transplanted. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21)
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Specification