Processes for obtaining microbial oil from microbial cells
First Claim
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1. A process for obtaining a microbial oil comprising one or more polyunsaturated fatty acids from one or more microbial cells, wherein the process comprises:
- (a) lysing the cells comprising the microbial oil to form a lysed cell composition;
(b) demulsifying the lysed cell composition to form a demulsified lysed cell composition;
(c) separating the oil from the demulsified lysed cell composition; and
(d) recovering the oil;
wherein (b) comprises adding an ionic emulsifier after step (a), and wherein (b) further comprises adding a salt selected from the group consisting of alkali metal salts, alkali earth metal salts, sulfate salts, and combinations thereof.
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Abstract
Disclosed are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition and then recovering the oil from the lysed cell composition. Further disclosed is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described.
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Citations
34 Claims
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1. A process for obtaining a microbial oil comprising one or more polyunsaturated fatty acids from one or more microbial cells, wherein the process comprises:
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(a) lysing the cells comprising the microbial oil to form a lysed cell composition; (b) demulsifying the lysed cell composition to form a demulsified lysed cell composition; (c) separating the oil from the demulsified lysed cell composition; and (d) recovering the oil; wherein (b) comprises adding an ionic emulsifier after step (a), and wherein (b) further comprises adding a salt selected from the group consisting of alkali metal salts, alkali earth metal salts, sulfate salts, and combinations thereof. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification