Photonic superlattice-based devices and compositions for use in luminescent imaging, and methods of using the same

• US 10,472,675 B2
• Filed: 07/13/2018
• Issued: 11/12/2019
• Est. Priority Date: 03/24/2016
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Dehlinger, Dietrich, Zhong, Cheng Frank, Topolancik, Juraj
• Primary Examiner(s): Porta, David P
• Assistant Examiner(s): Riddick, Blake C
• Application Number:
  US16/035,208
• Publication Number:
  US 20190024163A1
• Time in Patent Office: 487 Days
• Field of Search: 2504581, 2504591
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/168 Specific optical properties, e.g. reflective coatings
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6454 using an integrated detector array
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G02B 21/0076 arrangements using fluorescence or luminescence
• CPC Class Codes: G02B 21/18 Arrangements with more than one light path, e.g. for comparing two specimens
• CPC Class Codes: G02B 21/361 Optical details, e.g. image relay to the camera or image sensor G02B21/364 takes precedence; illumination details G02B21/06 and subgroups
• CPC Class Codes: G02B 5/20 Filters polarising elements G02B5/30

Abstract

Under one aspect, a device is provided for use in luminescent imaging. The device can include a photonic superlattice including a first material, the first material having a first refractive index. The first material can include first and second major surfaces and first and second pluralities of features defined through at least one of the first and second major surfaces, the features of the first plurality differing in at least one characteristic from the features of the second plurality. The photonic superlattice can support propagation of a first wavelength and a second wavelength approximately at a first angle out of the photonic superlattice, the first and second wavelengths being separated from one another by a first non-propagating wavelength that does not selectively propagate at the first angle out of the photonic superlattice.

Claims

1. A device comprising:

a photonic superlattice comprising a surface having wells of different sizes, the photonic superlattice supporting propagation of a first wavelength and a second wavelength, the photonic superlattice inhibiting propagation of a third wavelength between the first and second wavelengths; and

an optical component facing the surface, the optical component receiving first luminescence at the first wavelength at an angle, the optical component receiving second luminescence at the second wavelength at approximately the angle.

2. The device of claim 1, wherein the angle is approximately normal to the surface.

3. The device of claim 1, wherein the photonic superlattice comprises a first material that comprises the wells, the device further comprising a second material in contact with the first material.

4. The device of claim 3, wherein the second material has a higher refractive index than that of the first material.

5. The device of claim 3, further comprising a third material in contact with at least the second material, the third material comprising first and second luminophores, the first luminescence emitted by the first luminophore, the second luminescence emitted by the second luminophore.

6. The device of claim 5, wherein the first luminophore is coupled to a first nucleic acid, and wherein the second luminophore is coupled to a second nucleic acid.

7. The device of claim 1, wherein the wells include at least a first plurality of wells and a second plurality of wells, wherein each of the first plurality of wells has approximately a first size, and wherein each of the second plurality of wells has approximately a second size smaller than the first size.

8. The device of claim 7, wherein at least some of the first plurality of wells are positioned in a cross-shaped pattern, and wherein at least some of the second plurality of wells are positioned in corners of the cross-shaped pattern.

9. The device of claim 8, further comprising a plurality of posts, each of the plurality of posts positioned in a respective one of the first plurality of wells.

10. The device of claim 7, wherein at least some of the first plurality of wells are positioned in a circular pattern, and wherein at least some of the second plurality of wells are positioned around the circular pattern.

11. The device of claim 10, wherein at least one of the second plurality of wells is positioned inside the circular pattern.

12. The device of claim 10, further comprising a plurality of posts, each of the plurality of posts positioned in a respective one of the first plurality of wells.

13. The device of claim 1, further comprising a plurality of posts, each of the plurality of posts positioned in a respective one of the wells.

14. A method comprising:

receiving, by an optical component and at an angle from a photonic superlattice, first luminescence at a first wavelength, the photonic superlattice supporting propagation of the first wavelength and a second wavelength and comprising a surface having wells of different sizes; and

receiving, by the optical component and at approximately the angle from the photonic superlattice, second luminescence at the second wavelength, the photonic superlattice inhibiting propagation of a third wavelength between the first and second wavelengths.

15. The method of claim 14, further comprising coupling a first luminophore to a first polynucleotide, and coupling a second luminophore to a second polynucleotide, wherein the first luminescence is emitted by the first luminophore, and wherein the second luminescence is emitted by the second luminophore.

16. A composition comprising:

a photonic superlattice comprising a first material having a surface with wells of different sizes, the photonic superlattice supporting propagation of a first wavelength and a second wavelength, the photonic superlattice inhibiting propagation of a third wavelength between the first and second wavelengths; and

a second material disposed within, between, or over the wells, the second material comprising first and second luminophores, the first luminophore emitting first luminescence at the first wavelength at an angle, the second luminophore emitting second luminescence at the second wavelength at approximately the angle.

17. The composition of claim 16, wherein the wells include at least a first plurality of wells and a second plurality of wells, wherein each of the first plurality of wells has approximately a first size, and wherein each of the second plurality of wells has approximately a second size smaller than the first size.

18. The composition of claim 17, wherein at least some of the first plurality of wells are positioned in a cross-shaped pattern, and wherein at least some of the second plurality of wells are positioned in corners of the cross-shaped pattern.

19. The composition of claim 17, wherein at least some of the first plurality of wells are positioned in a circular pattern, and wherein at least some of the second plurality of wells are positioned around the circular pattern.

20. The composition of claim 16, further comprising a plurality of posts, each of the plurality of posts positioned in a respective one of the wells.