Method, system, and device for automating transfer of tape to microtome sections

• US 10,473,557 B2
• Filed: 06/10/2016
• Issued: 11/12/2019
• Est. Priority Date: 06/30/2015
• Status: Active Grant

• Current Assignee: Clarapath, Inc.
• Original Assignee: Clarapath, Inc.
• Inventors: Mitra, Partha P., Tolpygo, Alexander S., Kleinberg, Robert L.
• Primary Examiner(s): Whatley, Benjamin R
• Application Number:
  US15/179,916
• Publication Number:
  US 20170003309A1
• Time in Patent Office: 1,250 Days
• CPC Class Codes: G01N 1/06 providing a thin slice, e.g. microtome
• CPC Class Codes: G01N 2001/362 using continuous plastic film to mount sample
• CPC Class Codes: G01N 2035/00019 cassette structures
• CPC Class Codes: G01N 2035/00108 Test strips, e.g. paper
• CPC Class Codes: G01N 2203/0282 Two dimensional, e.g. tapes, webs, sheets, strips, disks or membranes
• CPC Class Codes: G01N 33/48764 Test tape taken off a spool
• CPC Class Codes: G01N 35/00009 provided with a sample supporting tape, e.g. with absorbent zones

Abstract

A system and method for automated transfer of a tape segment onto the face of a tissue block to be thin sectioned by microtomy includes applying, to a carrier strip, a plurality of serially-spaced patches of sample tape having an adhesive outer surface, transporting the carrier strip along a path adjacent to and spaced from the exposed sample surface to position one of the patches of sample tape adjacent to and covering the exposed sample surface, adhering the one of the patches of sample tape to the exposed sample surface.

Claims

1. An automated tape transport apparatus for delivering a tape to an exposed surface of a specimen, the tape transport apparatus comprising:

(a) a controller;

(b) a tape transport unit, wherein the controller is configured to control the tape transport unit to transport the tape for adhering the tape to the exposed surface of the specimen for adherence of a sample sectioned from the specimen in the automated apparatus, the tape transport unit having a supply reel at an upstream end and a take up reel at a downstream end, and the tape having a separable cover strip adhesively secured to the tape thereon;

(c) a specimen holding unit for mounting the specimen with the exposed surface of the specimen outwardly facing therefrom;

(d) a tape application unit, wherein the controller is configured to control the tape application unit to engage with the tape to apply a pressure to the tape to adhere the tape to the exposed surface after transport of the tape to a position adjacent and over the exposed surface, the tape application unit including a reciprocating actuator controlled by the controller and a sensor providing locational information to the reciprocating actuator, the tape application unit movable by the actuator from a first position to a second position closer to the specimen holding unit to apply the pressure on the tape and press the tape into contact with the specimen, the actuator moving the tape application unit away from the second position and away from the specimen holding unit after applying pressure to the tape;

(e) a specimen sectioning unit operable to section the exposed surface from the specimen onto the tape adhered to the specimen to create a sectioned sample; and

(f) a cover strip take up spool, wherein the controller is configured to control the cover strip take up spool to take up the cover strip after separation from the tape, the cover strip take up spool positioned downstream of the supply reel and spaced therefrom.

2. The tape transport apparatus of claim 1, further comprising a guide positioned between the specimen holding unit and the take-up reel and spaced from the tape application unit, wherein the controller is configured to control the guide to move the guide between a first position to apply a force to the tape enabling adherence of the tape to the sectioned sample and a second position wherein the guide is moved by the controller to move the tape to a second angle to move the tape away from the specimen holding unit, the guide engaging the tape at a region axially spaced from the specimen holding unit and a movable control is actuable by the controller to move the guide between the first and second positions.

3. The tape transport apparatus of claim 1, wherein the tape application unit includes a spring loaded roller to adhere the tape to the exposed surface of the specimen.

4. The tape transport apparatus of claim 1, further comprising the tape on the supply reel, wherein the tape includes a plurality of patches separably attached the tape, a patch of the plurality of patches adhering to the exposed surface of the specimen, and the controller is configured to advance the patches from the supply reel to the specimen holding unit.

5. The tape transport apparatus of claim 1, wherein the separated cover strip is directed along a path different than a path of the tape and the cover strip is on an inside surface of the supply reel when wound on the supply reel.

6. The tape transport apparatus of claim 4, wherein the cover strip covers the plurality of patches.

7. The tape transport apparatus of claim 4, wherein the plurality of patches are serially spaced along the tape.

8. The tape transport apparatus of claim 4, further comprising a first adherence between a first side of the tape and a first side of each patch of the plurality of patches, the first adherence is configured (i) to adhere the tape to the patch while the tape is rolled on a spool before use and while the tape is unwound from the spool in preparation for use, and (ii) to allow separation of the patch from the tape when the patch is positioned adjacent to and adhered to the exposed surface of the specimen, the cover strip covering the patch.

9. The tape transport apparatus of claim 8, further comprising a second adherence on a second side of each patch of the plurality of patches, the second side being opposite the first side of the patch of the plurality of patches, and the second adherence is configured (i) to minimize adhesion between the first side of the patch and the tape deeper within the spool before use;

and (ii) to adhere the patch to the exposed surface of the specimen but to allow the patch to pull off the exposed surface of the specimen without damage to the exposed surface after the exposed surface has been sectioned from the specimen and affixed to a microscope slide.

10. The tape transport apparatus of claim 9, wherein a first bond formed by the first adherence is stronger than a second bond formed by the second adherence.

11. The tape transport apparatus of claim 9, wherein the first adherence is an adhesive layer and the second adherence is an adhesive layer.

12. The tape transport apparatus of claim 1, wherein the cover strip is segmental.

13. The tape transport apparatus of claim 1, further comprising a tape guide axially spaced from the tape application unit, wherein the controller is configured to control the tape guide, wherein the position of the tape guide is movable by the controller from an apply position to a remove position, the tape guide being in the apply position during advancement of the tape to align with the specimen holding unit and remaining in the apply position during movement of the tape application unit to the second position by the controller.

14. The tape transport apparatus of claim 13, wherein when the tape guide is in the apply position, an angle formed by a first line between the specimen holding unit and the supply reel and a second line between the specimen holding unit and the tape guide is about 180 degrees, whereby the tape is adjacent to and aligned with the exposed surface of said specimen and when the tape guide is in the remove position, an angle formed by the first line between the specimen holding unit and the supply reel and the second line between the specimen holding unit and the tape guide is at an obtuse angle.

15. The tape transport apparatus of claim 1, wherein the controller comprises:

(a) a processor; and

(b) a non-transitory computer-readable medium encoding instructions for dispensing the tape so that a portion of the tape is adjacent to and covers the exposed surface of the specimen.

16. The tape transport apparatus of claim 1, further comprising a slide and a slide transfer station for transferring the sectioned sample from the tape to a slide.

17. The tape transport apparatus of claim 16, wherein the slide has an adhesive for adhering the sectioned sample to the slide.

18. The tape transport apparatus of claim 17, wherein the adhesive is catalyzed by ultra-violet light.

19. The tape transport apparatus of claim 17, wherein after curing, the tape is removed from the sectioned sample.

20. The tape transport apparatus of claim 2, wherein in the second position the tape is moved away from the specimen holding unit by the controller to separate the tape from a region of the tape adhered to the exposed surface of the specimen.