Methods for estimating photosynthetic characteristics in plant canopies and systems and apparatus related thereto

• US 10,473,592 B2
• Filed: 04/29/2016
• Issued: 11/12/2019
• Est. Priority Date: 04/29/2015
• Status: Active Grant

• Current Assignee: The Board of Trustees of Michigan State University (Michigan State University)
• Original Assignee: The Board of Trustees of Michigan State University (Michigan State University)
• Inventors: Kramer, David, Chen, Jin, Ostendorf, Elisabeth, Xu, Lei, Cruz, Jeffrey, Brodersen, Jeremy J.
• Primary Examiner(s): Green, Yara B
• Application Number:
  US15/569,570
• Publication Number:
  US 20180313760A1
• Time in Patent Office: 1,292 Days
• CPC Class Codes: A01G 7/00 Botany in general
• CPC Class Codes: G01N 2021/635 Photosynthetic material analysis, e.g. chrorophyll
• CPC Class Codes: G01N 2021/8466 Investigation of vegetal material, e.g. leaves, plants, fruits
• CPC Class Codes: G01N 21/3563 for analysing solids; Preparation of samples therefor
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

Methods of determining and characterizing photosynthesis in plant parts of one or more plants includes capturing a plurality of images of the plant parts of the one or more plants with a sensor are provided. Fluorescence of the plant parts of the one or more plants can be measured by storing a sensor image of observed fluorescence. Light absorbed by the plant parts of the one or more plants can be estimated by observing red and/or infrared reflectance of the plant parts. A characteristic of photosynthesis such as linear electron flow in plant parts of the one or more plants can be derived using the measured fluorescence of the plant parts, the reflectance and the light absorbed by the plant parts, and/or the three-dimensional model comprising the plant parts of the one or more plants. Related apparatus and systems are also provided.

Claims

1. A method of determining photosynthetic characteristics in one or more plants, comprising:

capturing a plurality of images of plant parts in said plants with one or more sensors, wherein the plurality of images comprises a first image comprising measurement of the fluorescence of the plant parts and a second image comprising capture of one or more reflectance images of the plant parts upon exposure to one or more lights; and

deriving information regarding a characteristic of photosynthesis of the plant parts by multiplying the plurality of images.

2. The method of claim 1, wherein one of the plurality of images is used to determine absorbance by the plant parts in said plant upon exposure to the light.

3. The method of claim 1, wherein the method further comprises capturing additional images wherein measuring and/or environmental conditions for capturing the additional images are altered from the measuring and/or environmental conditions for capturing the first image and the second image.

4. The method of claim 3, wherein the measuring conditions are selected from location of said sensors, number of said sensors, presence or absence of a filter on each of said sensors, number of said lights provided, quality of said lights provided, location(s) of said lights provided, and combinations thereof.

5. The method of claim 1, wherein the method further comprises providing infrared and/or red light to the plant parts and capturing the infrared and/or red reflectance images.

6. The method of claim 1, wherein measuring fluorescence of the plant parts comprises providing red light to the plant parts, removing the provided red light from the plant parts, and measuring the fluorescence of the plant parts promptly after removing the provided red light.

7. The method of claim 1, performed in a chamber, wherein the chamber contains one or more sensors, one or more lights, and one or more environmental controls, wherein the method further comprises using said environmental controls to control one or more of temperature, humidity, oxygen, carbon dioxide, and wind in the chamber.

8. The method of claim 1, further comprising one or more instruments, wherein the method further comprises using said instruments to measure time of flight from said instruments to the plant parts.

9. The method of claim 1, further comprising estimating the light absorbed by the plant parts by at least one of red light and infrared light reflectance images of the plant parts, wherein deriving a characteristic of photosynthesis of said plants further comprises multiplying the measured fluorescence of the plant parts by the estimated light absorbed by the plant parts.

10. The method of claim 1, wherein deriving a characteristic of photosynthesis comprises deriving one or more of a rate of photosynthesis, efficiency of photosynthesis, and linear electron flow (LEF) within the plant parts.

11. The method of claim 1, wherein the one or more sensors comprise one or more cameras and the one or more cameras comprise one or more filters mounted on said cameras.

12. The method of claim 1, wherein the plant parts comprise one or more leaves.

13. A method of estimating photosynthesis in a plurality of plants in a plant canopy, comprising:

capturing a plurality of images of said plants with one or more sensors, wherein said plant canopy is located in a chamber configured to simulate various weather conditions;

generating a three-dimensional model comprising plant parts of said plants from the plurality of images, wherein the three-dimensional model comprises one or more geometric parameters;

measuring fluorescence of the plant parts; and

deriving a characteristic of photosynthesis of the plant parts using the measured fluorescence of the plant parts and the three-dimensional model comprising the plant parts.

14. The method of claim 13, wherein deriving a characteristic of photosynthesis of said plants comprises mapping information from the measured fluorescence of the plant parts onto the three-dimensional model comprising the plant parts.

15. The apparatus of claim 13, wherein said images are captured in the presence of one or more red light sources, infrared light sources, actinic light sources, and/or measurement light sources.

16. A plant photosynthesis characterization apparatus, comprising:

one or more sensors configured to capture a plurality of images of a plurality of plants in a plant canopy located in a chamber configured to simulate various weather conditions; and

a computerized system coupled to receive the plurality of images of said plants from said sensors, wherein the computerized system is configured to generate a three-dimensional model of the plant parts from the received plurality of images, to measure fluorescence of the plant parts, and to derive a characteristic of photosynthesis of said plants using the measured fluorescence of the plant parts and the three-dimensional model of the plant parts.

17. The apparatus of claim 16, wherein said images are captured in the presence of one or more light sources selected from red light sources, infrared light sources, actinic light sources, and/or measurement light sources.

18. The apparatus of claim 17, further comprising a chamber, such that said sensors, said light sources, and said environmental controls are configured to control one or more of temperature, humidity, oxygen, carbon dioxide, and wind in the chamber.

19. The apparatus of claim 18, further comprising one or more instruments configured to measure time of flight from the instrument to the plant parts of said plants.

20. The apparatus of claim 19, wherein the computerized system is further configured to compensate for multiple layers of leaves in said plants by deriving a characteristic of photosynthesis of said plants.

21. The apparatus of claim 19, wherein the computerized system is further configured to estimate the light absorbed by the plant parts by at least one of red light and infrared light reflectance images of the plant parts, and the characteristic of photosynthesis derived comprises a multiple of the measured fluorescence of the plant parts with the estimated light absorbed by the plant parts.

22. The apparatus of claim 19, wherein the characteristic of photosynthesis derived comprises one or more of a rate of photosynthesis, efficiency of photosynthesis, and linear electron flow (LEF) within the plant parts.

23. The apparatus of claim 19, wherein said sensors comprise one or more cameras.

24. The apparatus of claim 19, wherein said cameras comprise one or more filters mounted on said cameras.

25. The apparatus of claim 19, wherein said plant parts comprises one or more leaves.

26. A non-transitory machine-readable medium with instructions stored thereon, the instructions when executed operable to cause a computerized system to:

capture a plurality of images of a plurality of plants via one or more sensors in a plant canopy located in a chamber configured to simulate various weather conditions;

generate a three-dimensional model comprising plant parts from the plurality of images;

measure fluorescence of the plant parts; and

derive a characteristic of photosynthesis of said plants using the measured fluorescence of the plant parts and the three-dimensional model comprising the plant parts.

27. The medium of claim 26, wherein the characteristic of photosynthesis derived comprises one or more of a rate of photosynthesis, efficiency of photosynthesis, and linear electron flow (LEF) within the plant parts.

28. The medium of claim 26, wherein said sensors comprise one or more cameras and said cameras have one or more filters mounted thereon.

29. The medium of claim 26, wherein said plant parts comprise one or more leaves.

30. The medium of claim 26, wherein said images are captured in the presence of one or more red light sources, infrared light sources, actinic light sources, and/or measurement light sources.

31. A system comprising:

a controller; and

a plant photosynthesis characterization apparatus in communication with the controller, wherein the apparatus comprises;

one or more sensors configured to capture a plurality of images of a plurality of plants in a plant canopy located in a chamber configured to simulate various weather conditions; and

a computerized system coupled to receive the one or more images of said plants from said sensors, the computerized system configured to generate a three-dimensional model of the plant parts from the received plurality of images, to measure fluorescence of the plant parts, and to derive a characteristic of photosynthesis of said plants using the measured fluorescence of the plant parts and the three-dimensional model comprising the plant parts.

32. The system of claim 31, wherein said images are captured in the presence of one or more red light sources, infrared light sources, actinic light sources, and/or measurement light sources.

33. A plant photosynthesis characterization apparatus, comprising:

one or more sensors configured to capture a plurality of images of a plurality of plants;

a chamber for said sensors, one or more light sources and one or more environmental controls, such that said sensors, said light sources, and said environmental controls are configured to control one or more temperature, humidity, oxygen, carbon dioxide, and wind in the chamber;

one or more instruments configured to measure time of flight from the instrument to the plant parts of said plants; and

a computerized system coupled to receive the plurality of images of said plants from said sensors, wherein the computerized system is configured to generate a three-dimensional model of the plant parts from the received plurality of images, to measure fluorescence of the plant parts, and to derive a characteristic pf photosynthesis of said plants using the measured fluorescence of the plant part and the three-dimensional model of the plant parts, wherein the computerized system is further configured to compensate for multiple layers of leaves in said plants by deriving a characteristic of photosynthesis of said plants.