Instruments and methods for the implantation of cell-seeded substrates

• US 10,478,206 B2
• Filed: 04/27/2012
• Issued: 11/19/2019
• Est. Priority Date: 04/29/2011
• Status: Active Grant

• Current Assignee: California Institute of Technology, Regents of the University of California (University of California), University of Southern California
• Original Assignee: University of Southern California
• Inventors: Humayun, Mark, Ahuja, Ashish, Tai, Yu-Chong, Grubbs, Robert, Brant, Rodrigo, Wells, Trent, Johnson, Lincoln Vallance, Hikita, Sherry T., Hinton, David
• Primary Examiner(s): Medway, Scott J
• Application Number:
  US14/114,160
• Publication Number:
  US 20150032207A1
• Time in Patent Office: 2,762 Days
• CPC Class Codes: A61B 17/2909 Handles
• CPC Class Codes: A61B 2017/2937 with flexible part
• CPC Class Codes: A61B 2017/2945 Curved jaws
• CPC Class Codes: A61B 2017/305 Tweezer like handles with tubular extensions, inner slidable actuating members and distal tools, e.g. microsurgical instruments
• CPC Class Codes: A61F 2/14 Eye parts, e.g. lenses, corneal implants; Implanting instruments specially adapted therefor; Artificial eyes
• CPC Class Codes: A61F 2/4601 for introducing bone substitute, for implanting bone graft implants or for compacting them in the bone cavity
• CPC Class Codes: A61F 9/0017 implantable in, or in contact with, the eye, e.g. ocular inserts
• CPC Class Codes: A61F 9/007 Methods or devices for eye surgery
• CPC Class Codes: A61F 9/00736 Instruments for removal of intra-ocular material or intra-ocular injection, e.g. cataract instruments A61F9/008 takes precedence
• CPC Class Codes: A61K 35/50 Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
• CPC Class Codes: A61L 2430/16 for reconstruction of eye parts, e.g. intraocular lens, cornea
• CPC Class Codes: A61L 27/16 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/3813 Epithelial cells, e.g. keratinocytes, urothelial cells
• CPC Class Codes: A61L 27/50 Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: A61M 2202/09 Body tissue
• CPC Class Codes: A61M 5/14 Infusion devices, e.g. infusing by gravity; Blood infusion; Accessories therefor
• CPC Class Codes: C08L 65/04 Polyxylenes
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 2533/30 : Synthetic polymers
• CPC Class Codes: C12N 2533/40 : Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 2533/50 : Proteins
• CPC Class Codes: C12N 5/0621 : Eye cells, e.g. cornea, iris pigmented cells photoreceptors C12N5/062

Abstract

Disclosed herein are instruments and methods for delivery of substrates, including cell-seeded substrates, to target tissues requiring treatment for various diseases that induce cell death, damage or loss of function. The substrates are configured to provide cells, including stem cells, with a structural support that allows interconnection with and transmission of biological signals between the cells and the target tissue.

Claims

1. An instrument for an implantation of a substrate into a target tissue of a subject, comprising:

a handpiece comprising a proximal end and a distal end, said distal end comprising an orifice;

an outer housing extending in a distal direction from said handpiece, a distal end of the outer housing terminating in a bevel;

an internal shaft configured to be longitudinally moveable within said outer housing and said handpiece;

forceps connected with the internal shaft;

a shaft movement control mechanism; and

a planar substrate having a tail and a body of a same thickness as the tail,

wherein activation of said movement control mechanism causes longitudinal movement of said internal shaft and forceps;

wherein movement of said internal shaft in a proximal direction is configured to cause said forceps to grasp the tail of the substrate, induce a rolling of the substrate against the bevel of said outer housing, and retract the substrate within said outer housing, and

wherein movement of said internal shaft in a distal direction is configured to cause the substrate to exit said outer housing and unroll and cause said forceps to open.

2. The instrument of claim 1, wherein said movement control mechanism comprises a slide mechanism.

3. The instrument of claim 1, wherein said movement control mechanism comprises a friction wheel mechanism.

4. The instrument of claim 1, wherein said movement control mechanism comprises one or more racks associated with said forceps and a gear mechanism that interacts with said racks.

5. The instrument of claim 1, further comprising an injection pathway.

6. The instrument of claim 5, wherein said injection pathway is configured to deliver a media from the handpiece to a target site at or near a distal-most portion of the internal shaft.

7. The instrument of claim 1, further comprising:

a substrate support platform with which said forceps are configured to reversibly interact.

8. The instrument of claim 7, wherein after interacting with the substrate support platform, proximal longitudinal movement of the movement control mechanism is configured to retract the internal shaft resulting in contact between outer edges of the substrate support platform and an inner portion of the outer housing, thereby inducing the substrate support platform to roll into a cylindrical shape.

9. The instrument of claim 8, wherein distal longitudinal movement of the movement control mechanism causes the outer edges of the substrate support platform to move distally beyond the outer housing, thereby allowing the rolled support platform to unroll.

10. The instrument of claim 1, wherein the substrate has a curved region between the tail and the body.

11. The instrument of claim 10, wherein a length of the tail is between 1 mm and 20 mm.

12. The instrument of claim 11, wherein a length of the tail is between 1 mm and 2 mm.

13. The instrument of claim 1, wherein a width of the tail is between 0.2 mm and 6 mm.

14. An instrument for an implantation of a substrate into a target tissue of a subject, comprising:

a handpiece comprising a proximal end and a distal end, said distal end comprising an orifice;

an outer housing extending in a distal direction from said handpiece, a distal end of the outer housing terminating in a bevel;

an internal shaft configured to be longitudinally moveable within said outer housing and said handpiece;

forceps connected with the internal shaft;

a shaft movement control mechanism; and

an injection pathway within the outer housing,

wherein activation of said movement control mechanism causes longitudinal movement of said internal shaft and forceps;

wherein movement of said internal shaft in a proximal direction is configured to cause said forceps to grasp a tail of the substrate, induce a rolling of the substrate against the bevel of said outer housing, and retract the substrate within said outer housing, and

wherein movement of said internal shaft in a distal direction is configured to cause the substrate to exit said outer housing and unroll and cause said forceps to open.

15. The instrument of claim 14, further comprising:

a substrate having a tail, a body, and a curved region between the tail and the body.

16. The instrument of claim 14, wherein said movement control mechanism comprises a slide mechanism.

17. The instrument of claim 14, wherein said movement control mechanism comprises a friction wheel mechanism.