Spatially encoded biological assays
DCFirst Claim
1. A method for determining a spatial location of a biological molecule of a tissue sample comprising(a) providing a plurality of beads, wherein the plurality of beads comprise a plurality of binding agents, wherein a binding agent of the plurality of binding agents (i) comprises a coding identifier having a nucleic acid sequence and (ii) is configured to interact with a biological molecule of a tissue sample, wherein the coding identifier corresponds to a location at which the binding agent interacts with a biological molecule of a tissue sample;
- (b) contacting the plurality of beads with the tissue sample such that the binding agent interacts with the biological molecule; and
(c) using the coding identifier to identify the location where the binding agent interacted with the biological molecule, thereby determining a spatial location of the biological molecule of the tissue sample.
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Abstract
The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.
401 Citations
30 Claims
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1. A method for determining a spatial location of a biological molecule of a tissue sample comprising
(a) providing a plurality of beads, wherein the plurality of beads comprise a plurality of binding agents, wherein a binding agent of the plurality of binding agents (i) comprises a coding identifier having a nucleic acid sequence and (ii) is configured to interact with a biological molecule of a tissue sample, wherein the coding identifier corresponds to a location at which the binding agent interacts with a biological molecule of a tissue sample; -
(b) contacting the plurality of beads with the tissue sample such that the binding agent interacts with the biological molecule; and (c) using the coding identifier to identify the location where the binding agent interacted with the biological molecule, thereby determining a spatial location of the biological molecule of the tissue sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification