Labelled nucleotides
DC CAFC
First Claim
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1. A nucleotide or nucleoside molecule having a ribose or deoxyribose sugar moiety and a base linked to a detectable label via a cleavable linker, wherein the sugar moiety comprises a protecting group attached via a 3′
- oxygen atom, and wherein said protecting group comprises an azido group that can be modified or removed to expose a 3′
OH group.
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Abstract
Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
144 Citations
62 Claims
-
1. A nucleotide or nucleoside molecule having a ribose or deoxyribose sugar moiety and a base linked to a detectable label via a cleavable linker, wherein the sugar moiety comprises a protecting group attached via a 3′
- oxygen atom, and wherein said protecting group comprises an azido group that can be modified or removed to expose a 3′
OH group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
- oxygen atom, and wherein said protecting group comprises an azido group that can be modified or removed to expose a 3′
-
9. A kit comprising a plurality of nucleotides, each having a ribose or deoxyribose sugar moiety and a base linked to a detectable label via a cleavable linker, wherein the sugar moiety comprises a protecting group attached via the 3′
- oxygen atom, and said protecting group comprises an azido group that can be modified or removed to expose a 3′
OH group, and wherein the detectable label linked to each nucleotide can be distinguished upon detection from the detectable labels used for structurally different nucleotides. - View Dependent Claims (10, 11, 12, 13)
- oxygen atom, and said protecting group comprises an azido group that can be modified or removed to expose a 3′
-
31. A nucleotide molecule, wherein:
-
the nucleotide molecule comprises an azidomethyl moiety attached to the 3′
oxygen atom such that the azidomethyl moiety prevents addition of a nucleotide to the 3′
oxygen atom,the nucleotide molecule comprises a 2′
deoxyribose, wherein said 2′
deoxyribose comprises a triphosphate at the 5′
position,the nucleotide molecule comprises a 7-deazaadenine base, the 7-deazaadenine base of the nucleotide molecule is substituted with a linker at the 7-deaza position, the linker is bound to a fluorophore, the linker comprises a cleavable moiety located between the 7-deazaadenine base and the fluorophore, the cleavable moiety comprises a disulfide moiety or an azido moiety, the cleavable moiety and the azidomethyl moiety are cleavable chemically under identical conditions, upon cleavage of the cleavable moiety, the fluorophore is removed from the nucleotide molecule and part of the linker remains attached to the 7-deazaadenine base, and upon cleavage of the azidomethyl moiety, a 3-OH group is formed and the formation of the 3-OH group allows addition of a nucleotide to the 3′
oxygen atom. - View Dependent Claims (32, 33)
-
-
34. A 7-substituted 7-deaza-2′
- -deoxy-adenosine triphosphate nucleotide molecule, wherein;
the 3′
-O is substituted with an azidomethyl moiety that prevents addition of a nucleotide to the 3′
-O,the 7-deazaadenine base of the molecule is linked to a fluorophore via a cleavable linker at the 7-deaza position of the base, the linker comprises a cleavable moiety located between the 7-deazaadenine base and the fluorophore, the cleavable linker and the azidomethyl moiety are cleavable in a chemical reaction under identical conditions, upon cleavage of the cleavable moiety, the fluorophore is removed from the molecule and part of the linker remains attached to the 7-deazaadenine base, and upon cleavage of the azidomethyl moiety, a 3′
-OH group is formed and the formation of the 3′
-OH group allows addition of a nucleotide to the 3′
-O.
- -deoxy-adenosine triphosphate nucleotide molecule, wherein;
- 35. A nucleotide molecule having the structure:
-
39. A nucleotide molecule, wherein:
-
the nucleotide molecule comprises an azidomethyl moiety attached to the 3′
oxygen atom such that the azidomethyl moiety prevents addition of a nucleotide to the 3′
oxygen atom,the nucleotide molecule comprises a 2′
deoxyribose, wherein said 2′
deoxyribose comprises a triphosphate at the 5′
position,the nucleotide molecule comprises a 7-deazaguanine base, the 7-deazaguanine base of the nucleotide molecule is substituted with a linker at the 7-deaza position, the linker is bound to a fluorophore, the linker comprises a cleavable moiety located between the 7-deazaguanine base and the fluorophore, the cleavable moiety comprises a disulfide moiety or an azido moiety, the cleavable moiety and the azidomethyl moiety are cleavable chemically under identical conditions, upon cleavage of the cleavable moiety, the fluorophore is removed from the nucleotide molecule and part of the linker remains attached to the 7-deazaguanine base, and upon cleavage of the azidomethyl moiety, a 3′
-OH group is formed and the formation of the 3′
-OH group allows addition of a nucleotide to the 3′
oxygen atom. - View Dependent Claims (40, 41)
-
-
42. A 7-substituted 7-deaza-2′
- -deoxy-guanosine triphosphate nucleotide molecule, wherein;
the 3′
-O is substituted with an azidomethyl moiety that prevents addition of a nucleotide to the 3′
-O,the 7-deazaguanine base of the molecule is linked to a fluorophore via a cleavable linker at the 7-deaza position of the base, the linker comprises a cleavable moiety located between the 7-deazaguanine base and the fluorophore, the cleavable linker and the azidomethyl moiety are cleavable in a chemical reaction under identical conditions, upon cleavage of the cleavable moiety, the fluorophore is removed from the molecule and part of the linker remains attached to the 7-deazaguanine base, and upon cleavage of the azidomethyl moiety, a 3′
-OH group is formed and the formation of the 3′
-OH group allows addition of a nucleotide to the 3′
-O.
- -deoxy-guanosine triphosphate nucleotide molecule, wherein;
- 43. A nucleotide molecule having the structure:
-
47. A nucleotide molecule, wherein:
-
the nucleotide molecule comprises an azidomethyl moiety attached to the 3′
oxygen atom such that the azidomethyl moiety prevents addition of a nucleotide to the 3′
oxygen atom,the nucleotide molecule comprises a 2′
deoxyribose, wherein said 2′
deoxyribose comprises a triphosphate at the 5′
position,the nucleotide molecule comprises a cytosine base, the cytosine base of the nucleotide molecule is substituted with a linker at the 5 position of the base, the linker is bound to a fluorophore, the linker comprises a cleavable moiety located between the cytosine base and the fluorophore, the cleavable moiety comprises a disulfide moiety or an azido moiety, the cleavable moiety and the azidomethyl moiety are cleavable chemically under identical conditions, upon cleavage of the cleavable moiety, the fluorophore is removed from the nucleotide molecule and part of the linker remains attached to the cytosine base, and upon cleavage of the azidomethyl moiety, a 3′
-OH group is formed and the formation of the 3′
-OH group allows addition of a nucleotide to the 3′
oxygen atom. - View Dependent Claims (48, 49)
-
-
50. A 5-substituted 2′
- -deoxy-cytidine triphosphate nucleotide molecule, wherein;
the 3′
-O is substituted with an azidomethyl moiety that prevents addition of a nucleotide to the 3′
-O,the cytosine base of the molecule is linked to a fluorophore via a cleavable linker at the 5 position of the base, the linker comprises a cleavable moiety located between the cytosine base and the fluorophore, the cleavable linker and the azidomethyl moiety are cleavable in a chemical reaction under identical conditions, upon cleavage of the cleavable moiety, the fluorophore is removed from the molecule and part of the linker remains attached to the cytosine base, and upon cleavage of the azidomethyl moiety, a 3′
-OH group is formed and the formation of the 3′
-OH group allows addition of a nucleotide to the 3′
-O.
- -deoxy-cytidine triphosphate nucleotide molecule, wherein;
- 51. A nucleotide molecule having the structure:
-
55. A nucleotide molecule, wherein:
-
the nucleotide molecule comprises an azidomethyl moiety attached to the 3′
oxygen atom such that the azidomethyl moiety prevents addition of a nucleotide to the 3′
oxygen atom,the nucleotide molecule comprises a 2′
deoxyribose, wherein said 2′
deoxyribose comprises a triphosphate at the 5′
position,the nucleotide molecule comprises a uracil base, the uracil base of the nucleotide molecule is substituted with a linker at the 5 position of the base, the linker is bound to a fluorophore, the linker comprises a cleavable moiety located between the uracil base and the fluorophore, the cleavable moiety comprises a disulfide moiety or an azido moiety, the cleavable moiety and the azidomethyl moiety are cleavable chemically under identical conditions, upon cleavage of the cleavable moiety, the fluorophore is removed from the nucleotide molecule and part of the linker remains attached to the uracil base, and upon cleavage of the azidomethyl moiety, a 3′
-OH group is formed and the formation of the 3′
-OH group allows addition of a nucleotide to the 3′
oxygen atom. - View Dependent Claims (56, 57)
-
-
58. A 5-substituted 2′
- -deoxy-uridine triphosphate nucleotide molecule, wherein;
the 3′
-O is substituted with an azidomethyl moiety that prevents addition of a nucleotide to the 3′
-O,the uracil base of the molecule is linked to a fluorophore via a cleavable linker at the 5 position of the base, the linker comprises a cleavable moiety located between the uracil base and the fluorophore, the cleavable linker and the azidomethyl moiety are cleavable in a chemical reaction under identical conditions, upon cleavage of the cleavable moiety, the fluorophore is removed from the molecule and part of the linker remains attached to the uracil base, and upon cleavage of the azidomethyl moiety, a 3′
-OH group is formed and the formation of the 3′
-OH group allows addition of a nucleotide to the 3′
-O.
- -deoxy-uridine triphosphate nucleotide molecule, wherein;
- 59. A nucleotide molecule having the structure:
Specification
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Litigation Data
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Current AssigneeIllumina Cambridge Limited (Illumina Incorporated)
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Original AssigneeIllumina Cambridge Limited (Illumina Incorporated)
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InventorsBalasubramanian, Shankar, Barnes, Colin Lloyd, Liu, Xiaohai, Milton, John, Wu, Xiaolin, Swerdlow, Harold
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Primary Examiner(s)Riley, Jezia
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Application NumberUS15/202,456Publication NumberTime in Patent Office1,232 DaysField of Search536 41, 536 231, 435 61US Class CurrentCPC Class CodesC07H 19/10 with the saccharide radical...C07H 19/20 with the saccharide radical...C07H 21/00 Compounds containing two or...C12P 19/34 Polynucleotides, e.g. nucle...C12Q 1/6806 Preparing nucleic acids for...C12Q 1/6818 involving interaction of tw...C12Q 1/6823 Release of bound markersC12Q 1/6834 Enzymatic or biochemical co...C12Q 1/6869 Methods for sequencingC12Q 1/6874 involving nucleic acid arra...C12Q 1/6876 Nucleic acid products used ...C12Q 2525/186 incorporating a non-extenda...
