Methods and systems for processing polynucleotides
First Claim
Patent Images
1. A method for multi-analyte processing or analysis, comprising:
- (a) providing a mixture comprising a first analyte, a second analyte, a labelling agent comprising a reporter oligonucleotide molecule that comprises a reporter barcode sequence associated with said labelling agent, and a support having coupled thereto(i) a first barcode molecule comprising a first oligonucleotide sequence configured to couple to said reporter oligonucleotide molecule, and(ii) a second barcode molecule comprising a second oligonucleotide sequence configured to couple to said second analyte,
wherein said second analyte is a different type of analyte than said first analyte,
wherein said first oligonucleotide sequence is different from said second oligonucleotide sequence, and
wherein said labelling agent is configured to couple to said first analyte;
(b) in said mixture, (i) using said reporter oligonucleotide molecule and said first oligonucleotide sequence of said first barcode molecule to synthesize a first barcoded product, and (ii) using said second analyte and said second oligonucleotide sequence of said second barcode molecule to synthesize a second barcoded product; and
(c) determining a sequence of (i) said first barcoded product or derivative thereof and (ii) said second barcoded product or derivative thereof, to identify said first analyte and/or said second analyte.
1 Assignment
0 Petitions
Accused Products
Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
-
Citations
58 Claims
-
1. A method for multi-analyte processing or analysis, comprising:
-
(a) providing a mixture comprising a first analyte, a second analyte, a labelling agent comprising a reporter oligonucleotide molecule that comprises a reporter barcode sequence associated with said labelling agent, and a support having coupled thereto (i) a first barcode molecule comprising a first oligonucleotide sequence configured to couple to said reporter oligonucleotide molecule, and (ii) a second barcode molecule comprising a second oligonucleotide sequence configured to couple to said second analyte,
wherein said second analyte is a different type of analyte than said first analyte,
wherein said first oligonucleotide sequence is different from said second oligonucleotide sequence, and
wherein said labelling agent is configured to couple to said first analyte;(b) in said mixture, (i) using said reporter oligonucleotide molecule and said first oligonucleotide sequence of said first barcode molecule to synthesize a first barcoded product, and (ii) using said second analyte and said second oligonucleotide sequence of said second barcode molecule to synthesize a second barcoded product; and (c) determining a sequence of (i) said first barcoded product or derivative thereof and (ii) said second barcoded product or derivative thereof, to identify said first analyte and/or said second analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
-
Specification