Method to produce chemical pattern in micro-fluidic structure
First Claim
1. A method of forming a flow cell, the method comprising:
- transferring an adhesive promoter onto one or both of a first substrate and a second substrate according to a pattern, wherein one or both of the first substrate and the second substrate are transparent;
transferring a bio-medium onto the adhesive promoter to form a capture site pattern;
forming a polymer layer on one or both of the first substrate and the second substrate; and
polymer bonding the first substrate to the second substrate such that the polymer layer extends between a surface of the first substrate and a surface of the second substrate.
3 Assignments
0 Petitions
Accused Products
Abstract
The present disclosure provides flow cells and methods of fabricating flow cells. The method includes combining three portions: a first substrate, a second substrate, and microfluidic channels between the first substrate and the second substrate having walls of a photoresist dry film. Through-holes for inlet and outlet are formed in the first substrate or the second substrate. Patterned capture sites are stamped on the first substrate and the second substrate by a nanoimprint lithography process. In other embodiments, parts of the patterned capture sites are selectively attached to a surface chemistry pattern formed of silicon oxide islands each disposed on an outcrop of a soft bottom layer.
-
Citations
20 Claims
-
1. A method of forming a flow cell, the method comprising:
-
transferring an adhesive promoter onto one or both of a first substrate and a second substrate according to a pattern, wherein one or both of the first substrate and the second substrate are transparent; transferring a bio-medium onto the adhesive promoter to form a capture site pattern; forming a polymer layer on one or both of the first substrate and the second substrate; and polymer bonding the first substrate to the second substrate such that the polymer layer extends between a surface of the first substrate and a surface of the second substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
Specification