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Imaging a target fluorophore in a biological material in the presence of autofluorescence

  • US 10,488,340 B2
  • Filed: 10/31/2017
  • Issued: 11/26/2019
  • Est. Priority Date: 09/29/2014
  • Status: Active Grant
First Claim
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1. A method for extracting an image of a target fluorophore in a biological material wherein a waveband for emission of the target fluorophore overlaps a waveband for emission of a second fluorophore in the biological material, the method comprising:

  • illuminating the biological material with a first excitation light to induce a first fluorescence emission arising from both fluorescence of the target fluorophore and fluorescence of the second fluorophore and with a second excitation light to induce a second fluorescence emission arising from only the fluorescence of the second fluorophore;

    acquiring a first fluorescence image from the first fluorescence emission and a second fluorescence image from the second fluorescence emission; and

    processing the first and second fluorescence images to extract a third fluorescence image representing the target fluorophore, wherein relative intensities of the first and second excitation lights are modulated prior to acquiring the first and second fluorescence images.

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