Isolation of bona fide pancreatic progenitor cells
First Claim
Patent Images
1. A method for isolating a cell population enriched for bona fide pancreatic progenitor cells, said method comprising:
- i) providing a cell population comprising at least one bona fide pancreatic progenitor cell, wherein the bona fide pancreatic progenitor cell expresses PDX1 and NKX6-1; and
ii) exposing said cell population to a ligand which binds to a marker specific for PDX1+ cells, wherein the marker is selected from the group consisting of;
FOLR1, GP2 and MPZ, and selecting the cells that bind to said ligand from the cells that do not bind to said ligand, thereby enriching the cell population for PDX1+ cells, wherein if the marker is GP2 or MPZ, then the PDX1+ cell population is also enriched for PDX1+ NKX6-1+ cells;
thereby obtaining a cell population enriched for bona fide pancreatic progenitor cells.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to a method for isolating bona fide pancreatic progenitor cells and to cell populations enriched for bona fide pancreatic progenitor cells.
28 Citations
20 Claims
-
1. A method for isolating a cell population enriched for bona fide pancreatic progenitor cells, said method comprising:
-
i) providing a cell population comprising at least one bona fide pancreatic progenitor cell, wherein the bona fide pancreatic progenitor cell expresses PDX1 and NKX6-1; and ii) exposing said cell population to a ligand which binds to a marker specific for PDX1+ cells, wherein the marker is selected from the group consisting of;
FOLR1, GP2 and MPZ, and selecting the cells that bind to said ligand from the cells that do not bind to said ligand, thereby enriching the cell population for PDX1+ cells, wherein if the marker is GP2 or MPZ, then the PDX1+ cell population is also enriched for PDX1+ NKX6-1+ cells;thereby obtaining a cell population enriched for bona fide pancreatic progenitor cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
Specification