Extraction of materials from regions of interest in a sample

• US 10,494,627 B2
• Filed: 03/08/2017
• Issued: 12/03/2019
• Est. Priority Date: 12/01/2014
• Status: Active Grant

• Current Assignee: Leica Microsystems CMS GmbH (Danaher Corporation)
• Original Assignee: General Electric Company
• Inventors: Nelson, John Richard, Gao, Wei, Puleo, Christopher Michael, Miller, Todd Frederick, Pitner, Christine Lynne, Shoudy, David Andrew, Corwin, Alex David
• Primary Examiner(s): Nagpaul, Jyoti
• Application Number:
  US15/453,719
• Publication Number:
  US 20170175105A1
• Time in Patent Office: 1,000 Days
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/06 Auxiliary integrated devices, integrated components
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: C12N 15/1013 by using magnetic beads
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 1/02 Devices for withdrawing samples sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08
• CPC Class Codes: G01N 1/10 in the liquid or fluent state burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 2001/028 Sampling from a surface, swabbing, vaporising
• CPC Class Codes: G01N 2001/4038 electric methods, e.g. electromigration, electrophoresis, ionisation

Abstract

A system for extracting material from a region of interest includes a fluid delivery base comprising an inlet channel and an outlet channel formed within the fluid delivery base, a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of the inlet channel and an open end of the outlet channel; a support comprising a sample-supporting surface facing the gasket and an opposing surface; and an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein one or both of the alignment member or the fluid delivery base are biased towards one another by a force (e.g., a magnet or spring force) and wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.

Claims

1. A method of extracting material from a region of interest, comprising:

aligning one or more fluid channels of a fluid delivery base with an alignment member to define a region of interest on a sample, wherein the fluid delivery base and the alignment member are positioned on opposing surfaces of the sample such that the fluid delivery base and the alignment member are separated from one another by at least the sample and wherein the aligning comprises moving the fluid delivery base into a magnetic field of the alignment member, or moving a magnetic field of the fluid delivery base towards the alignment member;

delivering extraction fluid to the sample via the one or more fluid channels; and

collecting the extraction fluid via the one or more fluid channels.

2. The method of claim 1, comprising emitting light from the fluid delivery base onto the sample before aligning the one or more fluid channels.

3. The method of claim 2, wherein the light is emitted from a fiber optic light channel disposed within the one or more fluid channels.

4. The method of claim 1, comprising isolating the region of interest of the sample via a gasket, wherein the gasket is affixed to the fluid delivery base.

5. The method of claim 4, wherein the gasket contacts the sample.

6. A system for extracting material from a region of interest, comprising:

a fluid delivery base comprising one or more channels formed within the fluid delivery base, wherein the fluid delivery base comprises a magnet;

a gasket affixed to the fluid delivery base, wherein the gasket comprises at least one opening exposing an open end of at least one of the one or more channels;

a support comprising a sample-supporting surface configured to hold a sample against the gasket and an opposing surface; and

an alignment member coupled to the opposing surface in a fixed position and such that the support is positioned between the fluid delivery base and the alignment member, wherein the fluid delivery base is separable from the support and configured to move along a plane of the sample-supporting surface to align with the alignment member.

7. The system of claim 6, wherein the gasket comprises a plurality of openings, and wherein the one or more channels comprise a plurality of channels, wherein each gasket opening is coupled to at least one of the one or more channels.

8. The system of claim 7, wherein the plurality of channels are fluidically coupled to one another.

9. The system of claim 6, wherein the system is coupled to an extraction device comprising an imager, and wherein the extraction device is configured to:

acquire an image of a sample using image acquisition circuit;

designate the one or more regions of interest on the sample;

and align the fluid delivery base on the sample such that the gasket forms a seal about the one or more regions of interest.

10. The system of claim 6, wherein one or both of the alignment member or the fluid delivery base comprises a magnet and wherein a pull force of a magnetic field of one or both of the fluid delivery base or the alignment member reversibly holds the fluid delivery base on the support.

11. The system of claim 6, wherein one or both of the alignment member or the fluid delivery base are biased towards one another with a spring force.

12. The system of claim 6, wherein, in operation, the gasket contacts the sample.

13. The system of claim 6, wherein the gasket is configured to be removable from the fluid delivery base.

14. The system of claim 6, comprising an adaptor comprising one or more interior channels that couple to the one or more channels of the fluid delivery base.

15. The system of claim 6, wherein the gasket is positioned between the fluid delivery base and the sample-containing surface.

16. The system of claim 6, wherein the fluid delivery base and the alignment member, when in operation, align along an axis, wherein the axis intersects the at least one opening of the gasket.

17. The system of claim 6, wherein the one or more channels are substantially parallel to each other.

18. A system for extracting a material from a region of interest of a sample, comprising:

a fluid delivery base comprising a fluid inlet channel and a fluid outlet channel fluidically coupled to the fluid inlet channel, the fluid inlet channel arranged to deliver an extraction fluid to an isolated portion of the sample corresponds to the region of interest, for extracting the material from the region of interest, and the fluid outlet channel arranged to collect the extraction fluid and the material extracted from the same region of interest;

a gasket affixed to a sample-facing surface of the fluid delivery base, the gasket configured to isolate the region of interest upon direct contact with the sample;

a support comprising a sample-supporting surface configured to hold the sample against the gasket and an opposing surface; and

an alignment member coupled to the opposing surface such that the support is positioned between the fluid delivery base and the alignment member, wherein the fluid delivery base is separable from the support and configured to align with the alignment member under magnetic force.