Spatially encoded biological assays
First Claim
Patent Images
1. A method of determining the presence or abundance of a plurality of biological molecules in a location of interest in a biological sample comprising:
- (a) contacting the biological sample with a first and a second probe for each of the plurality of biological molecules, wherein a first and second probe for each biological molecule each comprise a sequence that is complementary to a sequence in the biological molecule, and when the first and second probes are hybridized to the biological molecule, the first and second probes are capable of being ligated together;
(b) imaging the biological sample to identify the location of interest;
(c) ligating first and second probes that are hybridized to a biological molecule to generate a ligation product(s) having a sequence;
(d) separating the ligation product(s) from the location of interest in the biological sample; and
(e) determining the sequence of the separated ligation product(s), thereby determining the presence or abundance of the plurality of biological molecules at the location of interest in the biological sample,wherein step (b) is performed before step (d).
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.
-
Citations
29 Claims
-
1. A method of determining the presence or abundance of a plurality of biological molecules in a location of interest in a biological sample comprising:
-
(a) contacting the biological sample with a first and a second probe for each of the plurality of biological molecules, wherein a first and second probe for each biological molecule each comprise a sequence that is complementary to a sequence in the biological molecule, and when the first and second probes are hybridized to the biological molecule, the first and second probes are capable of being ligated together; (b) imaging the biological sample to identify the location of interest; (c) ligating first and second probes that are hybridized to a biological molecule to generate a ligation product(s) having a sequence; (d) separating the ligation product(s) from the location of interest in the biological sample; and (e) determining the sequence of the separated ligation product(s), thereby determining the presence or abundance of the plurality of biological molecules at the location of interest in the biological sample, wherein step (b) is performed before step (d). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
-
12. A method of determining the presence or abundance of a plurality of biological molecules in a location of interest in a biological sample comprising:
-
(a) imaging a biological sample to identify the location of interest; (b) contacting the location of interest in the biological sample with a first and a second probe for each of the plurality of biological molecules, wherein a first and second probe for each biological molecule each comprise a sequence that is complementary to a sequence in the biological molecule, and when the first and second probes are hybridized to the biological molecule, the first and second probes are capable of being ligated together; (c) ligating first and second probes that are hybridized to a biological molecule in the location of interest in the biological sample to generate a ligation product(s) having a sequence; (d) separating the ligation product(s) from the location of interest in the biological sample; and (e) determining the sequence of the separated ligation product(s), thereby determining the presence or abundance of the plurality of biological molecules in the location of interest in the biological sample. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
22. A method of determining the presence or abundance of a plurality of biological molecules in a location of interest in a biological sample comprising:
-
(a) imaging a biological sample to identify the location of interest; (b) contacting the location of interest in the biological sample with a first and a second probe for each of the plurality of biological molecules, wherein a first and second probe for each biological molecule each comprise a sequence that is complementary to a sequence in the biological molecule, and when the first and second probes are hybridized to the biological molecule, the first and second probes are capable of being ligated together; (c) ligating first and second probes that are hybridized to a biological molecule in the biological sample to generate a ligation product(s) having a sequence; (d) separating the ligation product(s) from the biological sample; (e) determining the sequence of the separated ligation product(s), thereby determining the presence or abundance of the plurality of biological molecules in the location of interest in the biological sample. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29)
-
Specification