Determining mechanical properties via ultrasound-induced resonance

• US 10,495,613 B2
• Filed: 07/05/2017
• Issued: 12/03/2019
• Est. Priority Date: 03/17/2015
• Status: Active Grant

• Current Assignee: Hemosonics LLC (Stago International SAS)
• Original Assignee: Hemosonics LLC (Stago International SAS)
• Inventors: Walker, William F., Corey, F. Scott, Homyk, Andrew, Higgins, Tim, Viola, Francesco, Regan, Frank, Ferrante, Elisa
• Primary Examiner(s): Saint Surin, Jacques M
• Application Number:
  US15/641,803
• Publication Number:
  US 20170363585A1
• Time in Patent Office: 881 Days
• Field of Search: 73571
• CPC Class Codes: A61B 8/0883 for diagnosis of the heart
• CPC Class Codes: A61B 8/485 involving measuring strain or elastic properties
• CPC Class Codes: A61B 8/5223 for extracting a diagnostic or physiological parameter from medical diagnostic data for algorithms to analyse biomedical images G06T7/0012
• CPC Class Codes: G01N 2291/014 Resonance or resonant frequency
• CPC Class Codes: G01N 2291/02466 Biological material, e.g. blood
• CPC Class Codes: G01N 2291/025 Change of phase or condition
• CPC Class Codes: G01N 2291/02818 Density, viscosity
• CPC Class Codes: G01N 2291/02827 Elastic parameters, strength or force
• CPC Class Codes: G01N 2291/0422 Shear waves, transverse waves, horizontally polarised waves
• CPC Class Codes: G01N 29/036 by measuring frequency or resonance of acoustic waves
• CPC Class Codes: G01N 29/222 Constructional or flow details for analysing fluids optoacoustic fluid cells G01N29/2425
• CPC Class Codes: G01N 29/4418 with a model, e.g. best-fit, regression analysis
• CPC Class Codes: G01N 29/4436 with a reference signal amplitude comparison G01N29/48
• CPC Class Codes: G01N 29/4472 Mathematical theories or simulation
• CPC Class Codes: G16H 50/30 for calculating health indices; for individual health risk assessment

Abstract

A device for estimating a mechanical property of a sample is disclosed herein. The device may include a chamber configured to hold the sample; a transmitter configured to transmit a plurality of waveforms, including at least one forcing waveform; and a transducer assembly operatively connected to the transmitter and configured to transform the transmit waveforms into ultrasound waveforms. The transducer assembly can also transmit and receive ultrasound waveforms into and out of the chamber, as well as transform at least two received ultrasound waveforms into received electrical waveforms. The device also includes a data processor that can receive the received electrical waveforms; estimate a difference in the received electrical waveforms that results at least partially from movement of the sample; and estimate a mechanical property of the sample by comparing at least one feature of the estimated difference to at least one predicted feature, wherein the at least one predicted feature is based on a model of an effect of the chamber wall. Finally, the device can also include a controller configured to control the timing of the ultrasound transmitter and data processor.

Claims

1. A device for evaluation of hemostasis, the device being configured for used with an acoustic interrogation system to measure at least one viscoelastic property of a test sample of blood, the device comprising:

a housing; and

a plurality of test chambers, each comprising a resonant portion, wherein the plurality of test chambers are each at least partially defined by the housing, wherein each of the plurality of test chambers is designed to hold a test sample of blood combined with a reagent or combination of reagent and to be interrogated by an acoustic interrogation system to determine a plurality of hemostatic parameters of the test samples,

wherein each resonant portion of a test chamber of the plurality of test chambers is configured to generate resonance from one or more acoustic pulses directed into the test chamber by the acoustic interrogation system, and wherein the each resonant portion is configured to generate a shear wave that is axisymmetric, in part, from applied acoustic radiation force exerted by the applied one or more acoustic pulses.

2. The device of claim 1, wherein the each resonant portion of the test chamber comprises a cylindrical geometry or a substantially cylindrical geometry so as to generate the resonance.

3. The device of claim 2, wherein the cylindrical region or the substantially cylindrical region has a diameter, and wherein the cylindrical region or the substantially cylindrical region has a length that terminates with a cap having the same diameter.

4. The device of claim 3, wherein the cap is hemispherical or substantially hemispherical.

5. The device of claim 3, wherein the cap has an interior convex surface.

6. The device of claim 3, wherein the cylindrical region or the substantially cylindrical region has an axis that aligns to that of the cap.

7. The device of claim 3, wherein the cylindrical region or the substantially cylindrical region is about 4.2 mm in diameter and about 1.5 mm in length.

8. The device of claim 1, wherein each the plurality of test chambers comprises a non-resonant portion, the non-resonant portion having varying diameters that are larger than that of the resonant portion.

9. The device of claim 1, wherein the resonant portion generates an ultrasound-induced resonance from the applied one or more acoustic pulses, wherein the applied one or more acoustic pulses are emitted as a plurality of set of pulses, wherein each of the set of pulses are emitted at a frequency range between about 2 kHz and about 16 kHz.

10. The device of claim 1, wherein the applied one or more acoustic pulses are directed through the test sample held within test chamber, and wherein the test chamber has an axisymmetric portion and has a major axis that is co-linear with a propagation vector of the applied one or more acoustic pulses.

11. The device of claim 1, wherein the test chamber is axisymmetric.

12. The device of claim 1, comprising:

an ultrasonic couplant to facilitate propagation of the applied one or more acoustic pulses transmitted into and returned from at least one of said plurality of test chambers.

13. The device of claim 1, comprising:

a focusing element configured to focus the applied one or more acoustic pulses transmitted into and returned from at least one of said plurality of test chambers.

14. The device of claim 13, wherein the focusing element forms a part of a given test chamber of the plurality of test chambers.

15. The device of claim 13, wherein a portion of the given test chamber of the plurality of test chamber is formed of a couplant coupled to the focusing element.

16. The device of claim 13, wherein the focusing element has a refractive shape.

17. The device of claim 13, wherein the applied one or more acoustic pulses comprise ultrasound waves, and wherein the focusing element has a curvature that focuses the ultrasound wave directed therethough.

18. The device of claim 1, wherein an interior surface of one or more of the plurality of test chambers is formed or treated so as to promote adhesion of a forming blood clot.

19. The device of claim 1, comprising:

an alignment feature associated with alignment of the device with one or more transducers of the acoustic interrogation system.

20. The device of claim 19, further comprising:

a chamber alignment feature associated with alignment of the plurality of test chambers, wherein the transducer alignment feature and the chamber alignment feature fit together to align the one or more transducer with the plurality of test chambers.

21. The device of claim 1, wherein the device is configured to be received by the acoustic interrogation system, and wherein the acoustic interrogation system comprises a transducer assembly configured to transmit the one or more acoustic pulses into the plurality of test chamber and to receive reflected acoustic pulses from the plurality of test chambers.

22. The device of claim 21, wherein the acoustic interrogation system is configured to maintain the device in a fixed position with respect to the transducer assembly.

23. The device of claim 1, wherein shear modulus is measured over time from an interrogation of the plurality of chambers by the acoustic interrogation system.

24. The device of claim 23, wherein the shear modulus of at least one test chamber is estimated by analyzing resonance of the shear wave generated within said test chamber.

25. The device of claim 1, wherein the evaluation of hemostasis comprises an assessment of coagulation factors that includes initiation of clotting, formation of fibrin mesh, platelets modulation factors, and clot dissolution.

26. The device of claim 1, wherein the resonant portion causes repeated reflections of an induced shear wave at walls defining portions of the resonant portion to generate resonance in the resonant portion.

27. The device of claim 1, wherein the housing is configured as a consumable cartridge.

28. The device of claim 1, wherein the test sample comprises clotted blood.