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Methods and compositions for integration of an exogenous sequence within the genome of plants

  • US 10,501,748 B2
  • Filed: 04/02/2014
  • Issued: 12/10/2019
  • Est. Priority Date: 04/05/2013
  • Status: Active Grant
First Claim
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1. A plant cell comprising a targeted genomic modification to one or more alleles of an endogenous acetohydroxyacid synthase (AHAS) gene in the plant cell, wherein:

  • (i) the endogenous AHAS gene comprises any one of SEQ ID NO;

    35-56 or 263-278;

    (ii) the plant cell comprises a site-specific nuclease that binds to a target site as shown in any of SEQ ID NO;

    35-56 or 263-278 and cleaves the endogenous AHAS gene; and

    (iii) the genomic modification comprises an insertion and/or deletion in the endogenous AHAS sequence of the plant cell within any of SEQ ID NO;

    37-56 or 263-278;

    between SEQ ID NO;

    35 and 36;

    between SEQ ID NO;

    37 and 39;

    between SEQ ID NO;

    38 and 39 or 40;

    between SEQ ID NO;

    41 and 42;

    between SEQ ID NO;

    43 and 45;

    between SEQ ID NO;

    44 and 45;

    between SEQ ID NO;

    46 and 47;

    between SEQ ID NO;

    48 and 29;

    between SEQ ID NO;

    50 and 51;

    between SEQ ID NO;

    52 or 53 and 54;

    between SEQ ID NO;

    55 and 56;

    between SEQ ID NO;

    263 and 264;

    between SEQ ID NO;

    265 and 266;

    between SEQ ID NO;

    267 and 268;

    between SEQ ID NO;

    269 and 270;

    between SEQ ID NO;

    271 and 272;

    between SEQ ID NO;

    273 and 274;

    between SEQ ID NO;

    275 and 276;

    or between SEQ ID NO;

    277 and 278 of the endogenous AHAS gene following cleavage of the endogenous AHAS gene by the site-specific nuclease.

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