Methods and compositions for integration of an exogenous sequence within the genome of plants
First Claim
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1. A plant cell comprising a targeted genomic modification to one or more alleles of an endogenous acetohydroxyacid synthase (AHAS) gene in the plant cell, wherein:
- (i) the endogenous AHAS gene comprises any one of SEQ ID NO;
35-56 or 263-278;
(ii) the plant cell comprises a site-specific nuclease that binds to a target site as shown in any of SEQ ID NO;
35-56 or 263-278 and cleaves the endogenous AHAS gene; and
(iii) the genomic modification comprises an insertion and/or deletion in the endogenous AHAS sequence of the plant cell within any of SEQ ID NO;
37-56 or 263-278;
between SEQ ID NO;
35 and 36;
between SEQ ID NO;
37 and 39;
between SEQ ID NO;
38 and 39 or 40;
between SEQ ID NO;
41 and 42;
between SEQ ID NO;
43 and 45;
between SEQ ID NO;
44 and 45;
between SEQ ID NO;
46 and 47;
between SEQ ID NO;
48 and 29;
between SEQ ID NO;
50 and 51;
between SEQ ID NO;
52 or 53 and 54;
between SEQ ID NO;
55 and 56;
between SEQ ID NO;
263 and 264;
between SEQ ID NO;
265 and 266;
between SEQ ID NO;
267 and 268;
between SEQ ID NO;
269 and 270;
between SEQ ID NO;
271 and 272;
between SEQ ID NO;
273 and 274;
between SEQ ID NO;
275 and 276;
or between SEQ ID NO;
277 and 278 of the endogenous AHAS gene following cleavage of the endogenous AHAS gene by the site-specific nuclease.
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Abstract
Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.
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Citations
16 Claims
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1. A plant cell comprising a targeted genomic modification to one or more alleles of an endogenous acetohydroxyacid synthase (AHAS) gene in the plant cell, wherein:
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(i) the endogenous AHAS gene comprises any one of SEQ ID NO;
35-56 or 263-278;(ii) the plant cell comprises a site-specific nuclease that binds to a target site as shown in any of SEQ ID NO;
35-56 or 263-278 and cleaves the endogenous AHAS gene; and(iii) the genomic modification comprises an insertion and/or deletion in the endogenous AHAS sequence of the plant cell within any of SEQ ID NO;
37-56 or 263-278;between SEQ ID NO;
35 and 36;
between SEQ ID NO;
37 and 39;
between SEQ ID NO;
38 and 39 or 40;
between SEQ ID NO;
41 and 42;
between SEQ ID NO;
43 and 45;
between SEQ ID NO;
44 and 45;
between SEQ ID NO;
46 and 47;
between SEQ ID NO;
48 and 29;
between SEQ ID NO;
50 and 51;
between SEQ ID NO;
52 or 53 and 54;
between SEQ ID NO;
55 and 56;
between SEQ ID NO;
263 and 264;
between SEQ ID NO;
265 and 266;
between SEQ ID NO;
267 and 268;
between SEQ ID NO;
269 and 270;
between SEQ ID NO;
271 and 272;
between SEQ ID NO;
273 and 274;
between SEQ ID NO;
275 and 276;
or between SEQ ID NO;
277 and 278 of the endogenous AHAS gene following cleavage of the endogenous AHAS gene by the site-specific nuclease. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification