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Polynucleotide modification methods

  • US 10,501,767 B2
  • Filed: 08/14/2014
  • Issued: 12/10/2019
  • Est. Priority Date: 08/16/2013
  • Status: Active Grant
First Claim
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1. A method for modifying a template double stranded polynucleotide, comprising:

  • (a) contacting a double stranded template polynucleotide with a MuA transposase and a population of double stranded MuA substrates each comprising at least one overhang of universal nucleotides, such that the transposase fragments the template polynucleotide into two or more double-stranded fragments, and ligates a substrate to an end of each of the double stranded fragments, thereby producing a plurality of fragment/substrate constructs; and

    (b) ligating the overhangs to the fragments in the constructs and thereby producing a plurality of modified double stranded polynucleotides.

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