Compositions and methods for detecting human pegivirus 2 (HPgV-2)
First Claim
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1. A composition comprising a synthetic DNA nucleic acid molecule,wherein said synthetic DNA nucleic acid molecule comprises a nucleotide sequence at least 12 nucleotides in length that hybridizes under stringent conditions to a portion of an HPgV-2 genomic sequence selected from the group consisting of:
- the NS2 gene, the 5′
UTR, the S gene, the E1 gene, the E2 gene, the NS4a gene, the NS4b gene, and the 3′
UTR, andwherein said genomic sequence of HPgV-2 is shown in SEQ ID NO;
1, 75, or 299-303.
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Abstract
Provided herein are compositions, methods, and kits for detecting human Pegivirus 2 (HPgV-2). In certain embodiments, provided herein are HPgV-2 specific nucleic acid probes and primers, and methods for detecting HPgV-2 nucleic acid. In other embodiments, provided herein are HPgV-2 immunogenic composition compositions, methods of treating a subject with immunogenic HPgV-2 peptides, and methods of detecting HPgV-2 subject antibodies in a sample.
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Citations
37 Claims
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1. A composition comprising a synthetic DNA nucleic acid molecule,
wherein said synthetic DNA nucleic acid molecule comprises a nucleotide sequence at least 12 nucleotides in length that hybridizes under stringent conditions to a portion of an HPgV-2 genomic sequence selected from the group consisting of: - the NS2 gene, the 5′
UTR, the S gene, the E1 gene, the E2 gene, the NS4a gene, the NS4b gene, and the 3′
UTR, andwherein said genomic sequence of HPgV-2 is shown in SEQ ID NO;
1, 75, or 299-303. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
- the NS2 gene, the 5′
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23. A composition comprising a synthetic nucleic acid molecule,
wherein said synthetic nucleic acid molecule comprises a nucleotide sequence at least 12 nucleotides in length that hybridizes under stringent conditions to a portion of an HPgV-2 genomic sequence selected from the group consisting of: - the NS2 gene, the 5′
UTR, the S gene, the E1 gene, the E2 gene, the NS4a gene, the NS4b gene, and the 3′
UTR, and wherein said genomic sequence of HPgV-2 is shown in SEQ ID NO;
1, 75, or 299-303;wherein the synthetic nucleic acid molecule is linked to a fluorescent label, chemiluminescent label or enzymatic label, a heterologous nucleic acid sequence or a solid support or the synthetic nucleic acid molecule comprises at least one modified base selected from the group consisting of;
phosphorothioate, boranophosphate, 4′
-thio-ribose, locked nucleic acid, 2′
-0-(2′
-methoxyethyl), 2′
-0-methyl, 2′
-fluoro, 2′
-deoxy-T-fluoro-b-D-arabinonucleic acid, phosphonoacetate, 2′
-3′
-seco-RNA, Morpholino nucleic acid analog, Peptide nucleic acid analog, phosphorodithioate, phosphoramidate, methylphosphonate, 4-acetylcytosine, 8-hydroxy-N6-methyladenosine, aziridinylcytosine, pseudoisocytosine, 5-(carboxyhydroxylmethyl)uracil, 5-fluorouracil, 5-bromouracil, 5-carboxymethylaminomethyl-2-thiouracil, 5-carboxymethylaminomethyluracil, dihydrouracil, inosine, N6-isopentenyladenine, 1-methyladenine, 1-methylpseudouracil, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-methyladenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxy-aminomethyl-2-thiouracil, beta-D-mannosylqueosine, 5′
-methoxycarbonylmethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid, oxybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, N-uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid, pseudouracil, queosine, 2-thiocytosine, 2,6-diaminopurine, 2-aminopurine, 5-amino-allyluracil, 5-hydroxymethylcytosine, 5-iodouracil, 5-nitroindole, 5-propynylcytosine, 5-propynyluracil, hypoxanthine, N3-methyluracil, N6,N6-dimethyladenine, purine, C-5-propynyl cytosine, C-5-propynyl uracil, and difluorotouyl. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
- the NS2 gene, the 5′
Specification
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Current AssigneeAbbott Laboratories, Regents of the University of California (University of California)
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Original AssigneeAbbott Laboratories
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InventorsChiu, Charles, Lee, Deanna, Berg, Michael, Dawson, George, Coller, Kelly, Cheng, Kevin, Hackett, Jr., John R., Frankel, Matthew, Forberg, Kenn
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Primary Examiner(s)Blumel, Benjamin P
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Application NumberUS15/893,120Publication NumberTime in Patent Office669 DaysField of SearchNoneUS Class CurrentCPC Class CodesA61K 39/12 Viral antigensA61K 49/085 conjugated systemsA61P 31/14 for RNA virusesC07K 14/005 from virusesC12N 2770/24021 Viruses as such, e.g. new i...C12N 2770/24022 New viral proteins or indiv...C12N 2770/24034 Use of virus or viral compo...C12Q 1/701 Specific hybridization probesG01N 2333/183 Flaviviridae, e.g. pestivir...G01N 2469/20 Detection of antibodies in ...G01N 33/56983 Viruses
