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Modified nucleotides

  • US 10,513,731 B2
  • Filed: 06/10/2016
  • Issued: 12/24/2019
  • Est. Priority Date: 08/23/2002
  • Status: Expired due to Term
First Claim
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1. A method of controlling the incorporation of a nucleotide having a deoxyribose sugar moiety into a growing polynucleotide that is complementary to a target single-stranded polynucleotide, the method comprising:

  • (a) incorporating the nucleotide into the growing complementary polynucleotide, thereby preventing introduction of subsequent nucleotide molecules into the growing polynucleotide, wherein the nucleotide comprises a fluorescent detectable label linked by a phosphine-cleavable azide-containing linker, and a protecting group is attached to the 3′

    oxygen atom of the deoxyribose sugar moiety; and

    (b) reacting the nucleotide with a water-soluble phosphine to cleave the linker and the protecting group in a single step.

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