Preparation of psilocybin, different polymorphic forms, intermediates, formulations and their use

• US 10,519,175 B2
• Filed: 10/09/2018
• Issued: 12/31/2019
• Est. Priority Date: 10/09/2017
• Status: Active Grant

• Current Assignee: Compass Pathfinder Limited (Compass Pathways Plc)
• Original Assignee: COMPASS Pathways Limited (Compass Pathways Plc)
• Inventors: Londesbrough, Derek John, Brown, Christopher, Northen, Julian Scott, Moore, Gillian, Patil, Hemant Kashinath, Nichols, David E.
• Primary Examiner(s): Anderson, Rebecca L
• Application Number:
  US16/155,386
• Publication Number:
  US 20190119310A1
• Time in Patent Office: 448 Days
• CPC Class Codes: A61K 47/36 Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
• CPC Class Codes: A61K 47/38 Cellulose; Derivatives thereof
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61K 9/2054 Cellulose; Cellulose derivatives, e.g. hydroxypropyl methylcellulose
• CPC Class Codes: C07B 2200/13 Crystalline forms, e.g. polymorphs
• CPC Class Codes: C07D 209/16 Tryptamines
• CPC Class Codes: C07F 9/5728 condensed with carbocyclic rings or carbocyclic ring systems

Abstract

This invention relates to the large-scale production of psilocybin for use in medicine. More particularly, it relates to a method of obtaining high purity crystalline psilocybin, particularly, in the form of Polymorph A. It further relates to a method for the manufacture of psilocybin and intermediates in the production thereof and formulations containing psilocybin.

Claims

1. A method of treating drug resistant depression comprising orally administering to a subject in need thereof a therapeutically effective amount of an oral dosage form, wherein, the oral dosage form comprises:

crystalline psilocybin in the form Polymorph A characterized by peaks in an XRPD diffractogram at 11.5, 12.0, 14.5, 17.5, and 19.7°

2θ

±

0.1°

2θ

, wherein the crystalline psilocybin has a chemical purity of greater than 97% by HPLC, and no single impurity of greater than 1%; and

silicified microcrystalline cellulose.

2. The method of claim 1, wherein the oral dosage form comprises 1 mg to 40 mg of crystalline psilocybin in the form Polymorph A.

3. The method of claim 2, wherein the oral dosage form comprises 5 mg of crystalline psilocybin in the form Polymorph A.

4. The method of claim 2, wherein the oral dosage form comprises 10 mg of crystalline psilocybin in the form Polymorph A.

5. The method of claim 2, wherein the oral dosage form comprises 25 mg of crystalline psilocybin in the form Polymorph A.

6. The method of claim 1, wherein the peak at 17.5°

2θ

±

0.1°

2θ

has a relative intensity compared to the peak at 14.5°

2θ

±

0.1°

2θ

of at least 5%.

7. The method of claim 1, wherein the peak at 17.5°

2θ

±

0.1°

2θ

has a relative intensity compared to the peak at 14.5°

2θ

±

0.1°

2θ

of at least 9%.

8. The method of claim 1, wherein the crystalline psilocybin is further characterized having either:

i. a water content of <

0.5% w/w;

or

ii. <

0.5% w/w loss in the TGA thermogram between ambient temperature.

9. The method of claim 1, wherein the crystalline psilocybin is a white to off white solid characterized by rod shaped crystals with a size range of 50 to 200 microns.

10. The method of claim 1, wherein the crystalline psilocybin is further characterized by an endothermic event in a DSC thermogram having a first onset temperature of between 145°

C. and 155°

C. and a second onset temperature of between 205 and 220°

C.

11. The method of claim 1, wherein the crystalline psilocybin is further characterized by one or more of the following:

a) a loss on drying of no more than 2% w/w;

b) residue on ignition of no more than 0.5% w/w;

c) assay (on a dry basis) of 95-103% by weight as measured by HPLC;

d) residual solvent content of no more than 3000 ppm methanol;

5000 ppm ethanol, 720 ppm THF, and 890 ppm toluene, as measured by HRGC;

e) phosphoric acid content of no more than 1% w/w as measured by ³¹P NMR; and

f) Inductively Coupled Plasma Mass Spectrometry (ICP-MS) elemental analysis, of;

i. no more than 1.5 ppm Cd

ii. no more than 1.5 ppm Pb

iii. no more than 4.5 ppm As

iv. no more than 9.0 ppm Hg

v. no more than 15 ppm Co

vi. no more than 30 ppm V

vii. no more than 60 ppm Ni

viii. no more than 165 ppm Li; and

ix. no more than 30 ppm Pd.

12. The method of claim 1, wherein a peak at 10.1°

2θ

±

0.1°

2θ

is absent or substantially absent.

13. The method of claim 1, wherein the peak at 10.1°

2θ

±

0.1°

2θ

has a relative intensity compared to the peak at 14.5°

2θ

±

0.1°

2θ

of less than 2%.

14. The method of claim 12, wherein the peak at 10.1°

2θ

±

0.1°

2θ

has a relative intensity compared to the peak at 14.5°

2θ

±

0.1°

2θ

of less than 1%.

15. The method of claim 12, wherein a peak at 10.1°

2θ

±

0.1°

2θ

is not detectable in the XRPD diffractogram.

16. The method of claim 1, wherein the crystalline psilocybin has no single impurity of greater than 0.5%.

17. The method of claim 1 wherein the oral dosage form comprises silicified microcrystalline cellulose with a particle size range from about 45 to 150 microns.

18. The method of claim 17, wherein the oral dosage form comprises a mixture of two silicified microcrystalline cellulose variants wherein the first variant has a particle size from about 45 to 80 microns and the second variant has a particle size of about 90 to 150 microns.

19. The method of claim 18, wherein about 30% or less of the microcrystalline cellulose is the first variant having a particle size from about 45 to 80 microns and about 70% or more of the microcrystalline cellulose is the second variant having a particle size of about 90 to 150 microns.

20. The method of claim 19, wherein about 20% or less of the microcrystalline cellulose is the first variant having a particle size from about 45 to 80 microns and about 80% or more of the microcrystalline cellulose is the second variant having a particle size of about 90 to 150 microns.

21. The method of claim 20, wherein about 15% or less of the microcrystalline cellulose is the first variant having a particle size from about 45 to 80 microns and about 85% or more of the microcrystalline cellulose is the second variant having a particle size of about 90 to 150 microns.