Integrated device for nucleic acid detection and identification
First Claim
1. A disposable platform for detecting a target nucleic acid, the disposable platform comprising:
- a sample chamber for receiving a sample comprising the target nucleic acid;
an amplification chamber connected via a first channel to said sample chamber and connected via a first vent channel to a first vent pocket;
a labeling chamber connected via a second channel to said amplification chamber and connected via a second vent channel to a second vent pocket;
a detection subsystem connected to said labeling chamber via a third channel and connected via a third vent channel to a third vent pocket;
a plurality of resistive heating elements; and
one or more temperature measuring devices;
wherein said vent pockets are separate from said sample chamber, said amplification chamber, and said detection subsystem such that said vent channels remain clear of fluid; and
wherein said vent pockets are each sealed from a lower pressure by a heat labile material located in a vicinity of one of said resistive heating elements; and
wherein said platform does not comprise a pumping mechanism for transporting the sample from one of said chambers to another of said chambers or to said detection subsystem.
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Accused Products
Abstract
A disposable assay platform for detecting a target nucleic acid comprising multiple chambers and a method for operating the assay platform. Solutions containing the target nucleic acid move from one chamber to the next chamber by opening a vent pocket. The resulting pressure change enables the solution to flow to the next chamber. The platform comprises an electronic layer and one or more fluid layers bonded together. All heating operations can be performed by using resistive heating elements in the platform. All cooling operations are preferably passive. The platform is preferably operated when in a vertical orientation and can be docked to an external docking station that controls the operation of the platform.
118 Citations
19 Claims
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1. A disposable platform for detecting a target nucleic acid, the disposable platform comprising:
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a sample chamber for receiving a sample comprising the target nucleic acid; an amplification chamber connected via a first channel to said sample chamber and connected via a first vent channel to a first vent pocket; a labeling chamber connected via a second channel to said amplification chamber and connected via a second vent channel to a second vent pocket; a detection subsystem connected to said labeling chamber via a third channel and connected via a third vent channel to a third vent pocket; a plurality of resistive heating elements; and one or more temperature measuring devices; wherein said vent pockets are separate from said sample chamber, said amplification chamber, and said detection subsystem such that said vent channels remain clear of fluid; and wherein said vent pockets are each sealed from a lower pressure by a heat labile material located in a vicinity of one of said resistive heating elements; and wherein said platform does not comprise a pumping mechanism for transporting the sample from one of said chambers to another of said chambers or to said detection subsystem. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 18)
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12. A method for detecting a target nucleic acid, the method consisting of:
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disposing a sample comprising the target nucleic acid in a sample chamber of a disposable platform; orienting the disposable platform vertically or at a tilt; reacting the sample with a liquid or previously lyophilized amplification reagent mix; opening a first vent pocket connected via a first vent channel to an amplification chamber to lower pressure, thereby enabling the reacted sample to flow into the amplification chamber; amplifying the target nucleic acid in the amplification chamber; opening a second vent pocket connected via a second vent channel to a labeling chamber to lower pressure, thereby enabling the amplified target nucleic acid to flow into the labeling chamber; labeling the amplified target nucleic acid using detection particles in the labeling chamber; opening a third vent pocket connected via a third vent channel to a detection subsystem to lower pressure, thereby enabling the labeled target nucleic acid to flow into the detection subsystem; and detecting the amplified target nucleic acid; wherein said vent pockets are separate from said amplification chamber, said labeling chamber, and said detection subsystem such that said vent channels remain clear of fluid; and wherein each opening step comprises rupturing a heat labile material sealing each vent pocket from lower pressure. - View Dependent Claims (13, 14, 15, 16, 17, 19)
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Specification