Detection method for Neisseria gonorrhoeae

• US 10,519,514 B2
• Filed: 09/17/2015
• Issued: 12/31/2019
• Est. Priority Date: 09/17/2014
• Status: Active Grant

• Current Assignee: Binx Health Limited
• Original Assignee: Atlas Genetics Limited
• Inventors: Filer, Danny, Ferrao, Claire, Chadwick, Sharon
• Primary Examiner(s): Salmon, Katherine D
• Application Number:
  US15/511,893
• Publication Number:
  US 20170260572A1
• Time in Patent Office: 1,566 Days
• Field of Search: None
• CPC Class Codes: C12Q 1/689 for bacteria
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: C12Q 2600/16 Primer sets for multiplex assays

Abstract

A method of detecting the presence of Neisseria gonorrhoeae in a sample. The method involves detecting a first target sequence taken from the NGO1642 gene and/or a second target sequence taken from the NGO1012 gene. The method may involve a step of amplifying the target sequence, and may involve hybridising the target sequence to a nucleic acid probe and identifying hybridisation. The method may involve simultaneous detection of other target sequences, e.g. from other pathogens.

Claims

1. A method of detecting the presence of Neisseria gonorrhoeae in a sample comprising:

amplifying a first target sequence comprising SEQ ID NO;

1 and a second target sequence comprising SEQ ID NO;

6;

hybridising the first target sequence to a nucleic acid probe comprising SEQ ID NO;

3 and a ferrocene label;

hybridising the second target sequence to a nucleic acid probe comprising SEQ ID NO;

8 and a ferrocene label; and

identifying the occurrence of hybridisation by detecting the state of the labelled probes, wherein said step of amplifying the first target sequence and the second target sequence uses the polymerase chain reaction and involves the use of forward primers consisting of SEQ ID NOs;

4 and 9 and reverse primers consisting of SEQ ID NOs;

5 and 10.