Apparatuses for heating microscope slides carrying specimens

• US 10,520,403 B2
• Filed: 08/13/2018
• Issued: 12/31/2019
• Est. Priority Date: 11/12/2008
• Status: Active Grant

• Current Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Original Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Inventors: Beer, Jonathan Peter
• Primary Examiner(s): Paschall, Mark H
• Application Number:
  US16/102,598
• Publication Number:
  US 20180348100A1
• Time in Patent Office: 505 Days
• Field of Search: 435404-406, 219494, 219385, 219486, 219506, 392382
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00138 Slides
• CPC Class Codes: G01N 2035/00168 Manufacturing or preparing test elements
• CPC Class Codes: G01N 2035/00346 Heating or cooling arrangements
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J

Abstract

A slide processing apparatus controls the temperature and orientation of a microscope slide carrying one or more specimens. The apparatus heats the specimen-bearing microscope slide while the slide is oriented to both facilitate adhesion between the specimens and the slide and control movement of the specimens relative to the microscope slide. A slide dryer of the apparatus conductively heats the specimens using a conductive slide heater that physically engages the microscope slide.

Claims

1. An apparatus for automatically processing microscope slides carrying specimens, the apparatus comprising:

a drying station including a slide dryer, wherein the drying station is configured to automatically hold the microscope slides at an angle of inclination to promote draining of liquid from the microscope slides, and wherein the slide dryer heats the microscope slides held at the angle of inclination such that a temperature along each of the microscope slides is higher than about 50 degrees Celsius to at least partially dry the specimens carried by the microscope slides;

a staining station that automatically delivers stains onto the microscope slides to stain the at least partially dried specimens;

a cover-slipping station that automatically places coverslips on the microscope slides;

a transport device that automatically transports the microscope slides between the drying station, the staining station, and the cover-slipping station; and

at least one controller programmed to

command the transport device to transport the microscope slides, which carry transfer liquid and the specimens, to the drying station,

command the drying station to position the microscope slides at the angle of inclination to drain the transfer liquid from the microscope slides, and

command the transport device to transport the microscope slides that have been dried in the drying station to the staining station.

2. The apparatus of claim 1, wherein the drying station comprises a slide handler configured to automatically position the microscope slides at the angle of inclination.

3. The apparatus of claim 2, wherein the slide handler comprises at least one of a slide manipulator, an X-Y-Z transport system, a robotic arm, or a pick and place robot.

4. The apparatus of claim 1, wherein the angle of inclination is greater than about 75 degrees.

5. The apparatus of claim 1, wherein the drying station is configured to hold the microscope slides at the angle of inclination in a predetermined spaced apart arrangement.

6. The apparatus of claim 1, wherein the transport device is configured to (1) transport the microscope slides from the drying station to the staining station after the specimens are at least partially dried, and (2) transport the microscope slides from the staining station to the cover-slipping station after the specimens are stained.

7. The apparatus of claim 1, wherein the transport device is configured to vertically move the microscope slides between the drying station, the staining station, and the cover-slipping station.

8. The apparatus of claim 1, wherein the transport device comprises a slide tray that carries the microscope slides.

9. An apparatus for automatically processing microscope slides carrying specimens, the apparatus comprising:

a drying station that automatically (1) holds the microscope slides at a substantially vertical orientation, and (2) heats the microscope slides held at the substantially vertical orientation to a temperature higher than about 50 degrees Celsius;

a processing station configured to automatically dispense one or more fluids to contact the specimens carried by the microscope slides;

a transport device configured to automatically transport the microscope slides between the drying station and the processing station; and

a controller programmed to

cause the transport device to transport the microscope slides carrying transfer liquid and the specimens to the drying station, and

cause the drying station to position the microscope slides at the substantially vertical orientation to promote draining of the transfer liquid from the microscope slides.

10. The apparatus of claim 9, wherein the drying station comprises a slide handler that automatically positions the microscope slides at the substantially vertical orientation.

11. The apparatus of claim 9, wherein the substantially vertical orientation comprises an angle of inclination greater than about 75 degrees.

12. The apparatus of claim 9, wherein the drying station is configured to heat the microscope slide by convection.

13. The apparatus of claim 12, wherein the drying station comprises a convection oven.

14. The apparatus of claim 9, wherein the one or more fluids comprise a stain.

15. An apparatus for processing microscope slides carrying specimens, the apparatus comprising:

a drying station that robotically positions the microscope slides at an inclined position, wherein the drying station automatically heats the microscope slides held at the inclined position such that a temperature along each of the microscope slides is higher than about 50 degrees Celsius to at least partially dry the specimens carried by the microscope slides;

a staining station configured to automatically stain the at least partially dried specimens;

a transport device configured to robotically transport the microscope slides between the drying station and the staining station; and

one or more controllers programmed to

command the transport device to move the microscope slides carrying transfer liquid and the specimens to the drying station, and

command the drying station to position the microscope slides at the inclined position to promote draining of the transfer liquid from the microscope slides.

16. The apparatus of claim 15, wherein the drying station comprises a slide handler configured to robotically position the microscope slides at the inclined position.

17. The apparatus of claim 15, wherein the microscope slides at the inclined position are held at an angle of inclination greater than about 75 degrees.

18. The apparatus of claim 15, wherein the drying station comprises a conduction slide dryer or a convection oven.

19. The apparatus of claim 15, wherein the transport device comprises a slide tray that carries the microscope slides.

20. The apparatus of claim 19, wherein the microscope slides are carried by the slide tray during transportation of the microscope slides between the drying station and the staining station.