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Nucleic acid sequences using tags

  • US 10,526,647 B2
  • Filed: 03/03/2017
  • Issued: 01/07/2020
  • Est. Priority Date: 11/09/2012
  • Status: Active Grant
First Claim
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1. A method for assembling a protein having a plurality of subunits, the method comprising:

  • a) providing a plurality of first subunits;

    b) providing a plurality of second subunits, wherein the second subunits are modified with respect to the first subunits;

    c) contacting the first subunits with the second subunits in a first ratio to form a plurality of proteins having the first subunits and the second subunits, wherein the plurality of proteins have a plurality of ratios of the first subunits to the second subunits; and

    d) fractionating the plurality of proteins using ion-exchange chromatography to enrich proteins that have a second ratio of the first subunits to the second subunits, wherein the second ratio is one second subunit per (n−

    1) first subunits, wherein ‘

    n’

    is the number of subunits comprising the protein.

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