Selective oxidation of 5-methylcytosine by TET-family proteins
DCFirst Claim
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1. A method for detecting a 5-methylcytosine residue in a nucleic acid, the method comprising:
- (a) oxidizing the 5-methylcytosine residue in the nucleic acid to generate a modified nucleic acid, wherein the oxidizing comprises contacting the nucleic acid with TET1, TET2, TET3, CXXC4, a catalytic fragment of any of these, or any combination thereof; and
(b)-detecting the modified nucleic acid, wherein detection of the modified nucleic acid is indicative of a presence of the 5-methylcytosine residue in the nucleic acid.
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Abstract
The present invention provides for novel methods for regulating and detecting the cytosine methylation status of DNA. The invention is based upon identification of a novel and surprising catalytic activity for the family of TET proteins, namely TET1, TET2, TET3, and CXXC4. The novel activity is related to the enzymes being capable of converting the cytosine nucleotide 5-methylcytosine into 5-hydroxymethylcytosine by hydroxylation.
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7 Claims
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1. A method for detecting a 5-methylcytosine residue in a nucleic acid, the method comprising:
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(a) oxidizing the 5-methylcytosine residue in the nucleic acid to generate a modified nucleic acid, wherein the oxidizing comprises contacting the nucleic acid with TET1, TET2, TET3, CXXC4, a catalytic fragment of any of these, or any combination thereof; and (b)-detecting the modified nucleic acid, wherein detection of the modified nucleic acid is indicative of a presence of the 5-methylcytosine residue in the nucleic acid. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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