System and method for isolating and analyzing cells
First Claim
1. A system for isolating cells in at least one of single-cell format and single-cluster format, comprising:
- a substrate having a broad surface;
an array comprising a set of wells defined at the broad surface of the substrate, each well of the set of wells including;
a base surface,an open surface directly opposing the base surface, defined at the broad surface of the substrate, and sized to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, anda first set of channels that fluidly couple each well to at least one adjacent well in the set of wells;
wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the first set of channels;
a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells, the fluid delivery module comprising an inlet fluidically coupled to each of the set of wells by a second set of channels, wherein each of the second set of channels comprises an identical length; and
a perimeter channel directly fluidly coupled to the inlet and to each well of the exterior subset of the set of wells, the exterior subset of the set of wells positioned at an outermost edge of the array.
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Accused Products
Abstract
A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.
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Citations
20 Claims
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1. A system for isolating cells in at least one of single-cell format and single-cluster format, comprising:
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a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well of the set of wells including; a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and sized to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a first set of channels that fluidly couple each well to at least one adjacent well in the set of wells; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the first set of channels; a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells, the fluid delivery module comprising an inlet fluidically coupled to each of the set of wells by a second set of channels, wherein each of the second set of channels comprises an identical length; and a perimeter channel directly fluidly coupled to the inlet and to each well of the exterior subset of the set of wells, the exterior subset of the set of wells positioned at an outermost edge of the array. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A system for isolating cells in single-cell format, comprising:
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an array comprising a first broad face and second broad face opposing the first broad face, the array defining; an array body bounded by a plane, the plane coplanar with the first broad face; a set of wells within the array body, each well of the set defining;
an aperture at the plane;
a base arranged between the aperture and the second broad face; and
a wall extending from the plane to the base; anda first set of channels defined within the array body, wherein each well of the set of wells is fluidly coupled to an adjacent well of the set by a respective channel of the first set of channels; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the first set of channels; a fluid delivery module surrounding the array, the fluid delivery module comprising an inlet fluidly coupled to each well of the set of wells by a second set of channels, wherein each channel of the second set of channels defines a substantially identical channel length; and a perimeter channel fluidly connected to the inlet and to each well of the exterior subset of the set of wells, the exterior subset of the set of wells positioned proximal an outermost edge of the array. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification