System and method providing intracoronary laser speckle imaging for the detection of vulnerable plaque

• US 10,534,129 B2
• Filed: 03/28/2008
• Issued: 01/14/2020
• Est. Priority Date: 03/30/2007
• Status: Active Grant

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Tearney, Guillermo J., Nadkarni, Seemantini K., Bouma, Brett Eugene
• Primary Examiner(s): Santos Rodriguez, Joseph M
• Application Number:
  US12/058,279
• Publication Number:
  US 20080262359A1
• Time in Patent Office: 4,309 Days
• Field of Search: 600476, 600478
• CPC Class Codes: A61B 1/00096 Optical elements
• CPC Class Codes: A61B 1/00167 Details of optical fibre bundles, e.g. shape or fibre distribution
• CPC Class Codes: A61B 5/0066 Optical coherence imaging
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/7285 for synchronising or triggering a physiological measurement or image acquisition with a physiological event or waveform, e.g. an ECG signal A61B6/5205, A61B6/541, A61B8/5207, A61B8/543 take precedence
• CPC Class Codes: G02B 6/06 the relative position of the fibres being the same at both ends, e.g. for transporting images
• CPC Class Codes: G02B 6/32 having lens focusing means positioned between opposed fibre ends with lens being an integral part of the single fibre end G02B6/262

Abstract

Apparatus and method according to an exemplary embodiment of the present invention can be provided for analyzing tissue. For example, the apparatus can include at least one first arrangement configured to illuminate at least one anatomical structure with at least one of at least one electro-magnetic radiation. The apparatus can also include at least one second arrangement that may include at least two wave-guiding arrangements associated with one another that are configured to receive a further electro-magnetic radiation reflected from the tissue and transmit at least one speckle pattern associated with the further electro-magnetic radiation. The wave-guiding arrangements may be structured so as to reduce crosstalk therebetween.

Claims

1. An apparatus for intracoronary laser speckle imaging, comprising:

a light-delivery optical fiber configured to illuminate a coronary wall with laser light that scatters from the coronary wall to form laser speckle patterns;

a light-collecting fiber bundle configured to transmit laser speckle patterns formed by the scattered light, wherein fibers in the light-collecting fiber bundle are separated by at least 3 times a width of at least one fiber core so as to reduce crosstalk between fibers down to no greater than 10 percent of collected light; and

a detector configured to detect the laser speckle patterns transmitted by the light-collecting fiber bundle;

wherein the apparatus is configured to diminish effects of intervening blood on detection of laser speckle patterns by allowing no more than 200 μ

m of blood to flow between the light-collecting fiber bundle and the coronary wall.

2. The apparatus of claim 1 wherein fibers in the light-collecting fiber bundle include a primary acid-resistant cladding and a secondary acid-soluble cladding that bonds individual fibers.

3. The apparatus of claim 1 wherein the light-collecting fiber bundle fiber bundle has a partial core size no greater than 0.36, wherein the partial core size is core area divided by individual fiber area.

4. The apparatus of claim 1 wherein the light-collecting fiber bundle is leached.

5. The apparatus of claim 1 wherein the crosstalk is measurable by illuminating one fiber in the light-collecting fiber bundle and detecting light leakage in fibers adjacent to the illuminated fiber.

6. The apparatus of claim 1 further including a catheter and a motorized, computer-controlled translation stage for translating the catheter along a coronary segment.

29. The apparatus of claim 1, wherein the system includes a fluid injection system configured to inject a fluid into the coronary wall to dilute the intervening blood.

30. The apparatus of claim 1, further including an expandable basket having a prong configured to reduce a separation between the light-collecting fiber bundle and the coronary wall down to no greater than 200 μ

m.

31. The apparatus of claim 30, wherein the prong houses the light-collecting fiber bundle, and wherein the prong includes an optical window through which speckle patterns are imaged.

7. A method for speckle imaging, comprising:

using a light-delivery optical fiber to illuminate an anatomical structure with coherent light that scatters from the anatomical structure to form a speckle pattern;

using a light-collecting fiber bundle to collect scattered light and transmit the speckle pattern formed by the scattered light, wherein fibers in the light-collecting fiber bundle are separated by at least 3 times a width of at least one fiber core so as to reduce crosstalk between fibers; and

to diminish effects of intervening blood on detection of the laser speckle pattern, allowing no more than 200 μ

m of whole blood to flow between the light-collecting fiber bundle and the anatomical structure.

8. The method of claim 7 wherein the light-collecting fiber bundle reduces a measurable crosstalk down to no greater than 10 percent of light collected by the light-collecting fiber bundle.

9. The method of claim 8 wherein the light-collecting fiber bundle includes cladding, and wherein the crosstalk is measurable by illuminating one fiber in the light-collecting fiber bundle and detecting light leakage in the cladding and fibers adjacent to the illuminated fiber.

10. The method of claim 8 wherein a radius of curvature of fibers in the light-collecting fiber bundle ranges from three centimeters to ten centimeters.

11. The method of claim 7 wherein one or more fibers in the light-collecting fiber bundle include a primary acid-resistant cladding and a secondary acid-soluble cladding that bonds individual fibers.

12. The method of claim 7 wherein the fiber bundle has a partial core size greater than 0.36, wherein the partial core size is core area divided by individual fiber area.

13. The method of claim 7 wherein the light-collecting fiber bundle is used for intracoronary laser speckle imaging.

14. The method of claim 7 wherein the fibers are situated within a contact-based intra-arterial catheter configured to reduce motion of a distal end of the fiber bundle during intracoronary imaging, and wherein the method further includes using the catheter for intracoronary imaging.

15. The method of claim 14 wherein the catheter is configured to reduce motion of a distal end of the fiber bundle during intracoronary laser speckle imaging.

16. The method of claim 14 wherein the catheter has a diameter no greater than 1.5 millimeters.

17. The method of claim 7 wherein the speckle imaging is intracoronary laser speckle imaging, and wherein the method further includes injecting a bolus of saline to dilute intervening blood and thereby diminish effects of the intervening blood on detection of the laser speckle pattern.

18. The method of claim 7 wherein the speckle imaging is intracoronary laser speckle imaging, and wherein the method further includes using a catheter with a motorized translation stage to control motion of the light-collecting fiber bundle along a coronary segment.

19. An apparatus for speckle imaging, comprising:

a light-delivery optical fiber configured to illuminate an anatomical structure with coherent light that scatters from the anatomical structure to form a speckle pattern; and

a light-collecting fiber bundle configured to collect scattered light and transmit the speckle pattern formed by the scattered light, wherein fibers in the light-collecting fiber bundle are separated by at least 3 times a width of at least one fiber core to reduce crosstalk between fiber;

wherein the apparatus is configured to diminish effects of intervening blood on detection of the laser speckle pattern by allowing no more than 200 μ

m of blood to flow between the light-collecting fiber bundle and the anatomical structure.

20. The apparatus of claim 19 wherein the light-collecting fiber bundle is further configured to reduce a measurable crosstalk down to no greater than 10 percent of collected light.

21. The apparatus of claim 20 wherein the light-collecting fiber bundle includes cladding, and wherein the crosstalk is measurable by illuminating one fiber in the light-collecting fiber bundle and detecting light leakage in the cladding and fibers adjacent to the illuminated fiber.

22. The apparatus of claim 19 further including a detector configured to detect and measure speckle patterns.

23. The apparatus of claim 19 wherein fibers in the light-collecting fiber bundle include a primary acid-resistant cladding and a secondary acid-soluble cladding that bonds individual fibers.

24. The apparatus of claim 19 wherein the fiber bundle has a partial core size no greater than 0.36, wherein the partial core size is core area divided by individual fiber area.

25. The apparatus of claim 19, wherein the fibers are situated within a contact-based intra-arterial catheter configured to maintain contact with vessel walls when the apparatus is imaging inside a blood vessel.

26. The apparatus of claim 25 wherein the catheter is configured to reduce motion of a distal end of the fiber bundle during intracoronary imaging.

27. The apparatus of claim 25 wherein the catheter has a diameter no greater than 1.5 millimeters.

28. The apparatus of claim 19 further including:

a catheter; and

a motorized, computer-controlled translation stage for translating the catheter along a coronary segment during intracoronary imaging.

32. A system for intracoronary laser speckle imaging, comprising:

a light-delivery optical fiber configured to illuminate a target confined by a coronary wall with laser light that scatters from the target to form a laser speckle pattern;

a light-collecting fiber bundle configured to transmit the laser speckle pattern formed by the scattered light;

a detector configured to detect the laser speckle pattern transmitted by the light-collecting fiber bundle; and

a means for diminishing effects of intervening blood on detection of laser speckle patterns by allowing no more than the optical equivalent of a 200 μ

m layer of whole blood, in diluted or undiluted form, to flow between the light-collecting fiber bundle and the coronary wall.

33. The system of claim 32, wherein the means for diminishing effects of intervening blood includes a fluid injection system configured to inject a fluid into the coronary wall to dilute the flowing blood.

34. The system of claim 32, wherein the means for diminishing effects of intervening blood includes an expandable basket having a prong configured to reduce a separation between the light-collecting fiber bundle and the coronary wall down to no greater than 200 μ

m.

35. The system of claim 34, wherein the prong houses the light-collecting fiber bundle, and wherein the prong includes an optical window through which speckle patterns are imaged.