Exposure system

• US 10,539,550 B2
• Filed: 03/19/2015
• Issued: 01/21/2020
• Est. Priority Date: 09/25/2012
• Status: Active Grant

• Current Assignee: Inhalation Sciences AB
• Original Assignee: Inhalation Sciences AB
• Inventors: Gerde, Per
• Primary Examiner(s): Saint-Vil, Eddy
• Assistant Examiner(s): Ermlick, William D
• Application Number:
  US14/662,363
• Publication Number:
  US 20150192566A1
• Time in Patent Office: 1,769 Days
• Field of Search: None
• CPC Class Codes: G01N 15/06 Investigating concentration of particle suspensions by weighing G01N5/00; investigating sedimentation of particle suspensions G01N15/04; investigating individual particles G01N15/10
• CPC Class Codes: G01N 15/075 by optical means
• CPC Class Codes: G01N 2015/0687 in solutions, e.g. non volatile residue
• CPC Class Codes: G01N 2500/04 Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
• CPC Class Codes: G01N 33/5026 on cell morphology
• CPC Class Codes: G01N 33/5044 involving specific cell types

Abstract

The invention relates to methodologies of obtaining a controlled exposure of an aerosol to a model material. The invention also provides an exposure cap and a system for studying or predicting the interaction between a model material, e.g. a cell, and an aerosolized agent. The controlled exposure of the aerosol to the model material, makes is possible to accurately calculate the mass-balance of the aerosol exposure.

Claims

1. A method of obtaining a controlled exposure of a model material to an aerosol comprising the steps of;

i. providing a container comprising the model material;

ii. inserting an exposure cap into said container so as to obtain a substantially sealed environment during the exposure;

iii. introducing a controlled aerosol flow to an upper part of the exposure cap and transporting the aerosol towards the surface of the model material with a flow rotating around a central axis of the exposure cap;

iv. admitting the aerosol to contact the model material, while shielding the model material container from aerosol contact; and

v. transporting remaining aerosol away from the model material surface in an axially upwards directed separate flow.

2. The method according to claim 1, comprising transporting the remaining aerosol from a single outlet in a lower part of the exposure cap with a single flow coinciding with the central axis of the model material container.

3. The method according to claim 1, comprising collecting remaining aerosol in exposure filters and detecting a concentration of aerosol introduced to the exposure cap.

4. The method according to claim 3, comprising estimating a deposited dose of the exposure from the detected aerosol concentration, aerosol deposited into the exposure filters and a calculated aerosol deposition factor.

5. The method according to claim 1, comprising contacting a model material resembling an airway-blood barrier with a perfusion fluid.

6. The method according to claim 5, wherein the aerosol comprises a test substance, wherein the method comprises determining a mass balance of a remaining test substance rinsed from the model material container and test substance accumulated in the perfusion fluid, thereby determining an initially deposited dose of the exposure.

7. The method according to claim 1, comprising admitting the aerosol contacting the model material with an essentially constant flow shear rate along the surface of the model material.

8. The method according to claim 1, wherein a flow rate of the aerosol decrease from the upper part of the exposure cap towards the surface of the model material, preferably by admitting a rotational flow around central axis in the upper part to transform to an axially downward flow towards the surface of the model material.