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Method of increasing proliferation of bone marrow mononuclear cells expressing STRO1-dim

  • US 10,544,394 B2
  • Filed: 08/27/2014
  • Issued: 01/28/2020
  • Est. Priority Date: 09/24/2004
  • Status: Active Grant
First Claim
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1. A method of increasing proliferation of STRO1dim cells, the method comprising:

  • co-culturing;

    a) greater than 5% STRO1bright multipotent mesenchymal cells isolated from bone marrow mononuclear cells withb) STRO1dim cells isolated from bone marrow mononuclear cellssuch that proliferation of the STRO1dim cells is increased,wherein;

    i) the STRO1bright multipotent mesenchymal cells comprise a plurality of cells expressing VCAM1,ii) the STRO1dim cells comprise more committed precursor cell types than the STRO1bright multipotent mesenchymal cells,iii) the STRO1bright cells are more primitive than the STRO1dim cells, andiv) the STRO1bright cells emit at least a four-fold greater fluorescent signal than STRO1dim cells as determined by FACS analysis.

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