Solid tumor methylation markers and uses thereof
First Claim
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1. A method of generating a methylation profile of a biomarker in a subject in need thereof, comprising:
- a) processing an extracted genomic DNA with a deaminating agent to generate a genomic DNA sample comprising deaminated nucleotides, wherein the extracted genomic DNA is obtained from a biological sample from the subject;
b) detecting a hybridization between the extracted genomic DNA and a probe, wherein the probe hybridizes to cg10673833 (myosin IG); and
c) generating a methylation profile based on the detected hybridization between the extracted genomic DNA and the probe;
wherein the probe comprises a structure of Formula I;
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Abstract
Disclosed herein are methods and kits for identifying a subject as having esophagus cancer, pancreatic cancer, or stomach cancer. Also provided herein are methods and kits for generating the methylation profile of a biomarker associated with esophagus cancer, pancreatic cancer, or stomach cancer.
53 Citations
26 Claims
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1. A method of generating a methylation profile of a biomarker in a subject in need thereof, comprising:
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a) processing an extracted genomic DNA with a deaminating agent to generate a genomic DNA sample comprising deaminated nucleotides, wherein the extracted genomic DNA is obtained from a biological sample from the subject; b) detecting a hybridization between the extracted genomic DNA and a probe, wherein the probe hybridizes to cg10673833 (myosin IG); and c) generating a methylation profile based on the detected hybridization between the extracted genomic DNA and the probe; wherein the probe comprises a structure of Formula I; - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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26. A method of selecting a subject suspected of having a solid tumor for treatment, the method comprising:
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a) processing an extracted genomic DNA with a deaminating agent to generate a genomic DNA sample comprising deaminated nucleotides, wherein the extracted genomic DNA is obtained from a biological sample from the subject suspected of having a solid tumor; b) generating a methylation profile comprising cg10673833 (myosin IG) from the extracted genomic DNA, wherein the generating comprises detecting a hybridization between the extracted genomic DNA and a probe that hybridizes to cg10673833 (myosin IG); c) comparing the methylation profile comprising cg10673833 with a control; d) identifying the subject as having the solid tumor if the methylation profile correlates to the control; and e) administering an effective amount of a therapeutic agent to the subject if the subject is identified as having the solid tumor; wherein the solid tumor is selected from esophagus cancer, pancreatic cancer, or stomach cancer; and wherein the probe comprises a structure of Formula I;
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Specification