Biosensor calibration structure containing different sensing surface area

• US 10,548,530 B2
• Filed: 03/01/2017
• Issued: 02/04/2020
• Est. Priority Date: 03/01/2017
• Status: Active Grant

• Current Assignee: International Business Machines Corporation
• Original Assignee: International Business Machines Corporation
• Inventors: Kinser, Emily R.
• Primary Examiner(s): Ball, J. Christopher
• Application Number:
  US15/446,782
• Publication Number:
  US 20180252662A1
• Time in Patent Office: 1,070 Days
• CPC Class Codes: A61B 2560/0223 of calibration, e.g. protocols for calibrating sensors
• CPC Class Codes: A61B 2562/0295 Strip shaped analyte sensors for apparatus classified in A61B5/145 or A61B5/157 disposable multilayered or laminated electrodes G01N27/307; details of handling chemical test elements G01N33/4875
• CPC Class Codes: A61B 2562/125 characterised by the manufacture of electrodes
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61B 5/6846 specially adapted to be brought in contact with an internal body part, i.e. invasive
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A biosensor calibration structure is provided that includes at least two electrode structures in which at least one of the electrode structures has a non-random nanopattern on the sensing surface which provides a different sensing surface area than at least one other electrode structure. The at least one other electrode structure may be non-patterned (i.e., flat) or have another non-random nanopattern on the sensing surface. A biological functionalization material such as, for example, glucose oxidase or glucose dehydrogenase, can be located on at least the sensing surface of each electrode structure. The biosensor calibration structure can be used within a biosensor calibration method.

Claims

1. A biosensor calibration structure comprising:

an array of electrode structures each having a sensing surface, wherein at least one of the electrode structures of the array of electrode structures has a non-random nanopattern on the sensing surface which provides a different sensing surface area than at least one other electrode structure in the array of electrode structures, wherein the at least one of the electrode structures having the non-random nanopattern comprises an electrode base structure having topography, wherein the electrode base structure comprises an electrically conductive material that differs from an electrically conductive material that comprises the topography.

2. The biosensor calibration structure of claim 1, wherein the at least one other electrode structure of the array of electrode structures is non-patterned.

3. The biosensor calibration structure of claim 1, wherein the topography comprises rods, cones, or annular structures.

4. The biosensor calibration structure of claim 1, wherein the at least one other electrode structure of the array of electrode structures has another non-random nanopattern on the sensing surface.

5. The biosensor calibration structure of claim 4, wherein the another non-random nanopattern of the at least one other electrode structure has a different density than the non-random nanopattern of the at least one of the electrode structures of the array of electrode structures.

6. The biosensor calibration structure of claim 4, wherein the another non-random nanopattern of the at least one other electrode structure has a different cross sectional size than the non-random nanopattern of the at least one of the electrode structures of the array of electrode structures.

7. The biosensor calibration structure of claim 4, wherein the another non-random nanopattern of the at least one other electrode structure has a different aspect ratio than the non-random nanopattern of the at least one of the electrode structures of the array of electrode structures.

8. The biosensor calibration structure of claim 1, further comprising a biological functionalization material located on at least the sensing surface of each electrode structure of the array of electrode structures.

9. The biosensor calibration structure of claim 8, wherein the biological functionalization material is composed of an oligonucleotide, a nucleic acid, a peptide, a ligand, a protein, an enzyme, or any other material apt to bind with a complementary target biomolecule.

10. The biosensor calibration structure of claim 9, wherein the biological functionalization material is composed of glucose oxidase or glucose dehydrogenase.

11. A calibration method comprising:

providing an array of electrode structures each having a sensing surface, wherein at least one of the electrode structures of the array of electrode structures has a non-random nanopattern on the sensing surface which provides a different sensing surface area than at least one other electrode structure in the array of electrode structures;

observing a signal generated by each electrode structure of the array of electrode structures in the presence of an analyte;

comparing each signal; and

computing an analyte concentration utilizing the comparison of signal data obtained from the sensing surface area of each of the electrode structures.

12. The calibration method of claim 11, wherein prior to observing the signal a biological functionalization material is formed on at least the sensing surface of each electrode structure of the array of electrode structures.

13. The calibration method of claim 12, wherein the biological functionalization material is composed of an oligonucleotide, a nucleic acid, a peptide, a ligand, a protein, an enzyme, or any other material apt to bind with a complementary target biomolecule.

14. The calibration method of claim 12, wherein the biological functionalization material is composed of glucose oxidase or glucose dehydrogenase.

15. The calibration method of claim 11, wherein the at least one other electrode structure of the array of electrode structures is flat.

16. The calibration method of claim 11, wherein the at least one other electrode structure of the array of electrode structures has another non-random nanopattern on the sensing surface, wherein the another non-random nanopattern of the at least one other electrode structure has a different density, different cross sectional size, or different aspect ratio than the non-random nanopattern of the at least one of the electrodes of the array of electrodes.