System for production of adenosine triphosphate

• US 10,550,415 B2
• Filed: 10/16/2008
• Issued: 02/04/2020
• Est. Priority Date: 10/17/2007
• Status: Active Grant

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Travis, Alexander
• Primary Examiner(s): Switzer, Juliet C
• Assistant Examiner(s): Martin, Paul C
• Application Number:
  US12/738,367
• Publication Number:
  US 20110059373A1
• Time in Patent Office: 4,128 Days
• Field of Search: 429401, 435174, 435175, 4352891, 435 92
• CPC Class Codes: C12P 19/32 having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide

Abstract

The present invention relates to a system for production of ATP. This system is comprised of a support and one or more enzymes coupled to that support which are capable of collectively producing ATP from glucose or fructose metabolism. The present invention is additionally directed to a device, which includes the system, and to a method for carrying out a reaction involving the conversion of ATP to ADP using the system.

Claims

1. A system comprising:

a support comprising a linking agent that binds a targeting domain and

a plurality of enzymes in the glycolytic pathway,

wherein said plurality of enzymes are positioned in the system to collectively produce adenosine triphosphate from glucose or fructose metabolism,

wherein said plurality of enzymes comprises hexokinase, glucose 6-phosphate isomerase, 6-phosphofructokinase, aldolase, triose-phosphate-isomerase, glyceraldehyde-3-phosphate-dehydrogenase, phosphoglycerate kinase, phosphoglycerate mutase, enolase, and pyruvate kinase,

wherein a germ cell-specific targeting/scaffolding domain of at least one enzyme of said plurality of enzymes has been replaced with the targeting domain that specifically binds to the linking agent of the support, wherein the at least one enzyme comprises hexokinase, glyceraldehyde 3-phosphate dehydrogenase, aldolase, or pyruvate kinase, and

wherein the system is contained within a device.

2. The system of claim 1, wherein the at least one enzyme of said plurality of enzymes in the glycolytic pathway is hexokinase and the targeting/scaffolding domain comprises the amino acid sequence of MGQNCQRGQAVDVEPKIRPPLTEE (SEQ ID NO:

6) or MGQICQRESATAAEKPKLHLLAES (SEQ ID NO;

     7).

3. The system of claim 1, wherein said support and said plurality of enzymes are encapsulated in an encapsulating agent.

4. The system of claim 3, wherein the encapsulating agent is selected from the group consisting of a liposome and silastic implant.

5. The system of claim 3, wherein the encapsulating agent comprises a silastic material.

6. The system of claim 3, wherein said support and said plurality enzymes are encapsulated in a single chamber defined by the encapsulating agent.

7. The system of claim 1, wherein the at least one enzyme is hexokinase.

8. The system of claim 1, wherein the at least one enzyme is glyceraldehyde 3-phosphate dehydrogenase.

9. The system of claim 1, wherein the at least one enzyme is aldolase.

10. The system of claim 1, wherein the at least one enzyme is pyruvate kinase.