×

Non-human mammals for the production of chimeric antibodies

  • US 10,555,506 B2
  • Filed: 04/11/2016
  • Issued: 02/11/2020
  • Est. Priority Date: 09/30/2008
  • Status: Active Grant
First Claim
Patent Images

1. A mouse whose genome comprises a transgene comprising an immunoglobulin heavy chain (IgH) locus, or a portion thereof, wherein said IgH locus comprises in operable linkage from 5′

  • to 3′

    an unrearranged DNA sequence of a human variable heavy (VH) gene segment, a diversity heavy (DH) gene segment, and a human joining heavy (JH) gene segment, operably linked to a constant region gene comprising a human CH1 exon, a human Cupper hinge exon, a human or mouse Cmiddle hinge exon, a mouse CH2 exon and a mouse CH3 exon, wherein the human CH1 exon is selected from the group consisting of a human mu constant region (Cμ

    ) CH1 exon, a human delta constant region (Cδ

    ) CH1 exon, a human gamma-1 constant region (Cγ

    l) CH1 exon, a human gamma-2 constant region (Cγ

    2) CH1 exon, a human gamma-4 constant region (Cγ

    4) CH1 exon, a human alpha constant region (Cα

    ) CH1 exon, and a human epsilon constant region (Cε

    ) CH1 exon, wherein the IgH locus further comprise an IgH non-coding region and wherein during B cell development, the IgH locus undergoes gene rearrangement and expresses a chimeric immunoglobulin heavy chain comprising a human heavy chain variable domain and a chimeric IgH constant domain.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×