Mechanically actuated vacuum controlled fluid collection

• US 10,562,022 B2
• Filed: 01/08/2018
• Issued: 02/18/2020
• Est. Priority Date: 12/05/2012
• Status: Active Grant

• Current Assignee: Pbc Renegadexbio
• Original Assignee: Boston Microfluidics, Inc.
• Inventors: Johnson, Brandon T., Christian, Kate E., Verner, Glenn H.
• Primary Examiner(s): Eom, Robert J
• Application Number:
  US15/864,051
• Publication Number:
  US 20180154351A1
• Time in Patent Office: 771 Days
• CPC Class Codes: A61B 10/007 for taking urine samples measuring urological functions A61B5/20; genital receptacles A61F5/451; urinals for bed-ridden persons A61G9/006
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0832 cylindrical, tube shaped
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00

Abstract

A mechanically-actuated vacuum-controlled fluid collection system includes a mechanically-actuated vacuum controller (MA VC) to draw fluid into a chamber through the opening to the chamber. The system may include a releasable seal to seal the opening, and the MAVC may include a spring-loaded plunger to create a vacuum within the chamber when sealed. The system includes multiple fluid chambers, and may further include a single actuator or multiple corresponding actuators. The system may be configured to add a pre-loadable reagent to fluid drawn into the one or more chambers, and may be configured to add the reagent in proportion to a volume of the fluid. The system may be controllable to release collected fluid to another device, such as for assay and/or transport. The system may be configured to draw a liquid biological sample such as urine, and may include a fluid interface to draw fluid from a biological sample container.

Claims

1. A fluid collection device, comprising:

a housing having a fluid chamber configured to receive, measure and retain a fluid and an opening to the fluid chamber;

a mechanically-actuated, non-adjustable vacuum controller to draw in a fixed, predetermined amount of the fluid into the fluid chamber through the opening, the predetermined amount of fluid being fixed by either a part of the housing, or a part disposed within the housing, the part thereby limiting an extent to which the vacuum controller draws in fluid;

the mechanically-activated vacuum controller being further configured to thereby store the fixed, predetermined volume of fluid in the fluid chamber, as the fixed, predetermined volume of fluid is drawn sequentially through the opening and then into the fluid chamber by the mechanically-actuated vacuum controller; and

the fluid chamber further configured to hold the fixed, predetermined amount of fluid for transport within the device.

2. The device of claim 1 additionally comprising:

a reagent chamber, the reagent chamber disposed within the housing, and preloaded with a fixed, predetermined amount of a reagent prior to activation of the mechanically-actuated, non-adjustable vacuum controller.

3. The device of claim 2 wherein the reagent chamber is configured to hold a fixed, predetermined amount of a liquid reagent.

4. The device of claim 2, wherein the fluid chamber has multiple sealable fluid chambers to receive a collection of mixed fluid and reagent samples.

5. The device of claim 2, wherein the mechanically-actuated, non-adjustable vacuum controller is configured to add reagent in proportion to volumes of multiple portions of measured fluid.

6. The device of claim 2, wherein the reagent chamber is preloaded with a liquid reagent.

7. The device of claim 2, wherein the reagent chamber is preloaded with a dry reagent.

8. The device of claim 2, wherein mixed fixed, predetermined amounts of fluid and reagent stored within the fluid chamber are only accessible by opening the housing.

9. The device of claim 2, further including:

a mechanical actuator to actuate the mechanically-actuated vacuum controller, wherein a portion of the mechanical actuator extends through a surface of the housing to permit a user to actuate the mechanically-actuated vacuum controller; and

wherein the reagent and the mechanically-actuated vacuum controller are pre-disposed within the housing, inaccessible to the user.

10. The device of claim 1, wherein the mechanically-actuated, non-adjustable vacuum controller is configured to draw a biological sample fluid into the fluid chamber through the opening.

11. The device of claim 1, wherein the mechanically-actuated, non-adjustable vacuum controller further includes a push-button based actuator to actuate the mechanically-actuated, non-adjustable vacuum controller.

12. The device of claim 1, further including a fluid stopper, disposed within or on the housing, to releasably seal the opening to the fluid chamber for transport of the fixed, predetermined amount of fluid.

13. The device of claim 12, wherein the mechanically-actuated, non-adjustable vacuum controller includes a plunger to create a vacuum within the fluid chamber when the fluid stopper is engaged to seal the opening.

14. The device of claim 13, wherein the mechanically-actuated, non-adjustable vacuum controller includes a spring to apply a mechanical force to the plunger.

15. The device of claim 14, wherein the mechanically-actuated, non-adjustable vacuum controller further comprises a push button to trigger the mechanically-actuated, non-adjustable vacuum controller to draw fluid into the fluid chamber through the opening, the push button having an opening through which the plunger passes when the push button is triggered.

16. The device of claim 1, wherein the mechanically-actuated, non-adjustable vacuum controller is configured to draw in and retain multiple fixed, predetermined volumes of fluid into the fluid chamber sequentially or in parallel.

17. The device of claim 1, wherein the fixed, predetermined amount of fluid is only accessible by opening the housing.

18. The device of claim 1, further including one or more of a pierce-able membrane and rubber septum disposed over the opening.

19. The device of claim 1, configured to interface with a diagnostic or transport system.

20. The device of claim 1 wherein the mechanically-actuated vacuum controller further comprises:

a plunger to create a vacuum within the fluid chamber; and

a mechanical actuator to actuate the plunger, wherein the mechanical actuator is connected to the plunger to control a position of the plunger,

wherein the part of the housing, or the part disposed within the housing, thereby controls movement of the plunger to only move by a fixed, non-adjustable, predetermined distance within the fluid chamber, and

wherein the mechanical actuator is predisposed within the housing, inaccessible to a user of the device.