Systems and methods for automatic plate washing

• US 10,562,032 B2
• Filed: 05/26/2017
• Issued: 02/18/2020
• Est. Priority Date: 05/26/2017
• Status: Active Grant

• Current Assignee: Aushon BiosyStems, Inc. (Quanterix Corporation)
• Original Assignee: Aushon BiosyStems, Inc. (Quanterix Corporation)
• Inventors: Honkanen, Peter, Bradbury, David
• Primary Examiner(s): Lorenzi, Marc
• Application Number:
  US15/606,881
• Publication Number:
  US 20180339296A1
• Time in Patent Office: 998 Days
• CPC Class Codes: B01L 13/02 for receptacle or instruments
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 9/523 for multisample carriers, e.g. used for microtitration plates
• CPC Class Codes: B08B 3/022 Cleaning travelling work B08B3/042 takes precedence
• CPC Class Codes: B08B 3/024 Cleaning by means of spray elements moving over the surface to be cleaned
• CPC Class Codes: B08B 9/00 Cleaning hollow articles by methods or apparatus specially adapted thereto B08B6/00 takes precedence
• CPC Class Codes: B08B 9/093 by the force of jets or sprays B08B9/0813 , B08B9/20 take precedence

Abstract

The present disclosure relates to automated systems and methods for washing microtiter plates that offer advantages such as increased efficiency and decreased contamination during the washing process. In an exemplary embodiment, an automated system for washing multiwell plates comprises an arm having a metal portion and having a multiwell plate holder for holding a multiwell plate; a first rotational servo configured to rotate the multiwell plate about a first axis; a second rotational servo configured to rotate the arm about a second axis; and a controller configured to operate the system to dispels fluid in the multiwell plate.

Claims

1. An automated system for washing a multiwall plate, the automated system comprising:

an arm having a metal portion and having a multiwell plate holder for holding the multiwell plate;

a first rotational servo configured to rotate the multiwell plate about a first axis;

a second rotational servo configured to rotate the arm about a second axis; and

a first electromagnet;

a first stop;

a fluid manifold adapted to dispense a wash fluid into a corresponding plurality of wells in the multiwell plate; and

a controller configured to;

operate the first rotational servo as a first closed loop servo to rotate the multiwell plate about the first axis from an upward position to a downward position;

operate the second rotational servo as a second closed loop servo to rotate the arm about the second axis such that the metal portion moves towards the first electromagnet and to transition the second rotational servo to operating as an open loop servo; and

activate the first electromagnet to cause the first electromagnet to pull the metal portion towards the first electromagnet while the second rotational servo operates as the open loop servo, wherein a deceleration impulse caused by the arm contacting the first stop dispels fluid in the multiwell plate.

2. The automated system for washing a multiwell plate of claim 1, wherein the first stop comprises the first electromagnet.

3. The automated system for washing a multiwell plate of claim 2, wherein the first electromagnet pulls the metal portion towards the first electromagnet and holds the metal portion against the first electromagnet.

4. The automated system for washing a multiwell plate of claim 1, wherein the metal portion comprises a metal plate.

5. The automated system for washing a multiwell plate of claim 1, further comprising at least one of a removal basin and an absorbent material, wherein the deceleration impulse caused by the arm contacting the first stop dispels fluid from the multiwell plate into the at least one of the removal basin and the absorbent material.

6. The automated system for washing multiwell plate of claim 5, wherein the automated system comprises both the removal basin and the absorbent material, and wherein the automated system further comprises:

a second electromagnet;

a second stop; and

a linear servo configured to move the arm between the removal basin and the absorbent material;

wherein the controller is further configured to;

operate the second rotational servo as the second closed loop servo to rotate the arm about the second axis such that the metal portion of the arm moves towards the second electromagnet and transition the second rotational servo to operating as the open loop servo; and

activate the second electromagnet to pull the metal portion of the arm towards the second electromagnet while the second rotational servo operates as the open loop servo, wherein a deceleration impulse caused by the arm contacting the second stop dispels fluid from the multiwell plate into at least the other of the absorbent material and the removal basin.

7. The automated system for washing a multiwell plate of claim 1, wherein the controller activates the first electromagnet 20 to 40 milliseconds before the arm contacts the first stop.

8. The automated system for washing a multiwell plate of claim 6, wherein the absorbent material is placed on top of a support for the absorbent material.

9. The automated system for washing a multiwell plate of claim 8, further comprising a source wheel that supplies clean absorbent material to the support.

10. The automated system for washing a multiwell plate of claim 8, further comprising a take-up wheel that removes used absorbent material from the support.

11. The automated system for washing a multiwell plate of claim 1, wherein the fluid manifold has a base and a plurality of dispense needles adapted to dispense the wash fluid into the corresponding plurality of wells in the multiwell plate, the fluid manifold configured to dispense the wash fluid across rows of columns of the multiwell plate.

12. The automated system for washing a multiwell plate of claim 1, wherein the arm is configured to move along a linear axis allowing movement of the multiwell plate underneath the fluid manifold.