Automated system and method for advancing tape to transport cut tissue sections

• US 10,571,368 B2
• Filed: 04/01/2017
• Issued: 02/25/2020
• Est. Priority Date: 06/30/2015
• Status: Active Grant

• Current Assignee: Clarapath, Inc.
• Original Assignee: Clarapath, Inc.
• Inventors: Zhang, Cong, Fasciano, Mark, Mitra, Partha P.
• Primary Examiner(s): Whatley, Benjamin R
• Application Number:
  US15/476,983
• Publication Number:
  US 20170205317A1
• Time in Patent Office: 1,060 Days
• CPC Class Codes: G01N 1/06 providing a thin slice, e.g. microtome
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2001/061 Blade details
• CPC Class Codes: G01N 2035/00019 cassette structures
• CPC Class Codes: G01N 35/00009 provided with a sample supporting tape, e.g. with absorbent zones

Abstract

An automated tape transfer apparatus including a tape feed mechanism feeding a continuous length of an adhesive tape through the automated tape transfer apparatus and a tape applicator applying the adhesive tape to a cutting face of a sample block, wherein a section of the sample block is adhered to the adhesive tape after cutting of the section from the sample block. A slide station transfers the cut section from the adhesive tape to a slide.

Claims

1. An automated method for advancing a tape comprising the following automated controller controlled steps:

pressing a first portion of a continuous length of an adhesive tape to a first cutting face of a sample block so the adhesive tape contacts and adheres to and covers the first cutting face;

after adherence of the tape to the first cutting face, cutting a first section from the sample block;

moving the first portion of the adhesive tape away from the sample block after the first section has been cut from the sample block, wherein the first section is adhered to the first portion of the adhesive tape and the cutting exposes a second cutting face of the sample block;

pressing a second portion of the adhesive tape to the second cutting face of the sample block;

after adherence of the tape to the second cutting face and covering of the second cutting face, cutting a second section from the sample block;

moving the second portion of the adhesive tape away from the sample block after a second section has been cut from the sample block, wherein the second section is adhered to the second portion of the adhesive tape;

moving the first and second portions of the adhesive tape carrying the corresponding first and second sections to a slide station, the slide station supporting at least first and second slides; and

pressing the first section to the first slide in the slide station to transfer the first section to the first slide and pressing the second section to the second slide in the slide station to transfer the second section to the second slide, wherein the step of pressing the first section includes moving a roller or a cam within a slide station to press the first section on the first slide, the slide movable in an axial direction and the roller movable from a first position axially spaced from the first slide to a second position over the first slide to press the first section on the first slide; and

wherein the foregoing steps are all performed by an automated controller controlled apparatus.

2. The method of claim 1, wherein the first and second slides are moved along a conveyor to a position to enable sequential transfer of the first section to the first slide and the second section to the second slide in the slide station.

3. The method of claim 1, further comprising the step of controlling a distance between the first portion and second portion of the adhesive tape.

4. The method of claim 1, further comprising the step of controlling one or more of a speed, acceleration and jerk of moving operations.

5. The method of claim 1, wherein the step of pressing the first portion of the adhesive tape to the first cutting face includes pressing the roller member or the cam that holds the adhesive tape in a direction against the cutting face throughout an entire length of the cutting face.

6. The method of claim 1, wherein the step of pressing the first portion of the adhesive tape fully covers the first cutting face with an adhesive region of the adhesive tape prior to the step of cutting the first section.

7. The method of claim 1, wherein the sample block comprises a tissue sample enclosed in a supporting block of paraffin.

8. The method of claim 1, further comprising the step of heating, cooling or applying UV light to the tape to change the properties of the tape to lower an adhesive strength of the tape subsequent to the step of transferring the first section to the first slide and prior to a step of removing the tape from the first section on the slide.

9. The method of claim 1, further comprising a take-up mechanism that takes up the adhesive tape after the adhesive tape has exited the slide station.

10. The method of claim 1, further comprising the step of pre-coating with a UV-curable adhesive the first and second slides in the slide station.

11. The method of claim 1, wherein the method further comprises the step of applying UV-curable adhesive and activating the adhesive by applying a UV light source positioned below the slides.

12. The method of claim 1, further comprising the step of imaging the sample block via an optical device during the automated method to assess a condition of the cutting of the sample block.

13. The method of claim 1, further comprising the step of providing one or more optical sensors or imaging devices to provide feedback on a position and a quality of the first section and second section on the adhesive tape.

14. The method of claim 1, wherein the slide station contains a plurality of slides arranged in an array.

15. The method of claim 9, wherein the slide station contains a plurality of slides arranged in an array and the take up mechanism is downstream of the slide station.

16. An automated method for advancing a tape comprising the following automated controller controlled steps:

pressing a first portion of a continuous length of an adhesive tape to a first cutting face of a sample block so the adhesive tape contacts and adheres to and covers the first cutting face;

after adherence of the tape to the first cutting face, cutting a first section from the sample block;

moving the first portion of the adhesive tape away from the sample block after the first section has been cut from the sample block, wherein the first section is adhered to the first portion of the adhesive tape and the cutting exposes a second cutting face of the sample block;

pressing a second portion of the adhesive tape to the second cutting face of the sample block;

after adherence of the tape to the second cutting face and covering of the second cutting face, cutting a second section from the sample block;

moving the second portion of the adhesive tape away from the sample block after a second section has been cut from the sample block, wherein the second section is adhered to the second portion of the adhesive tape;

moving the first and second portions of the adhesive tape carrying the corresponding first and second sections to a slide station, the slide station supporting at least first and second slides;

pressing the first section to the first slide in the slide station to transfer the first section to the first slide and pressing the second section to the second slide in the slide station to transfer the second section to the second slide;

wherein the foregoing steps are all performed by an automated controller controlled apparatus and

indicating via an optical device during the automated method that the tape has been adhered to the cutting face prior to the step of cutting the first section from the sample block wherein the first section is cut by a microtome.

17. An automated method for advancing a tape comprising the following automated controller controlled steps:

pressing a first portion of a continuous length of an adhesive tape to a first cutting face of a sample block so the adhesive tape contacts and adheres to and covers the first cutting face;

after adherence of the tape to the first cutting face, cutting a first section from the sample block;

moving the first portion of the adhesive tape away from the sample block after the first section has been cut from the sample block, wherein the first section is adhered to the first portion of the adhesive tape and the cutting exposes a second cutting face of the sample block;

pressing a second portion of the adhesive tape to the second cutting face of the sample block;

after adherence of the tape to the second cutting face and covering of the second cutting face, cutting a second section from the sample block;

moving the second portion of the adhesive tape away from the sample block after a second section has been cut from the sample block, wherein the second section is adhered to the second portion of the adhesive tape;

moving the first and second portions of the adhesive tape carrying the corresponding first and second sections to a slide station, the slide station supporting at least first and second slides; and

pressing the first section to the first slide in the slide station to transfer the first section to the first slide and pressing the second section to the second slide in the slide station to transfer the second section to the second slide, wherein the step of pressing the first section includes moving a roller within the slide station to press the first section on the first slide, the roller movable by linear movement of a translation portion of the slide station to release the first section from the tape, the roller moving substantially parallel to a longitudinal axis of the slide;

wherein the foregoing steps are all performed by an automated controller controlled apparatus.

18. The method of claim 17, wherein the step of moving the roller moves the roller linearly parallel to an upper surface of the slide and the roller applies a constant force on the adhesive tape during application of the first section to the first slide.