Analyte-sensing device

• US 10,575,765 B2
• Filed: 10/13/2015
• Issued: 03/03/2020
• Est. Priority Date: 10/13/2014
• Status: Active Grant

• Current Assignee: GluSense Ltd.
• Original Assignee: GluSense Ltd.
• Inventors: Brill, Boaz
• Primary Examiner(s): Winakur, Eric F
• Assistant Examiner(s): Fardanesh, Marjan
• Application Number:
  US15/517,318
• Publication Number:
  US 20170303838A1
• Time in Patent Office: 1,603 Days
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14556 by fluorescence A61B5/14555 takes precedence
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 2201/021 Special mounting in general

Abstract

An implantable unit includes fluorescent sensor molecules, each of which includes a binding site for an analyte, a donor fluorophore, and an acceptor fluorophore; and a first light source. An external system includes an external reading unit, which includes a light sensor; and a processor, which is configured to (i) during a first time period: (a) drive the first light source to generate light having a first illumination peak wavelength appropriate for excitation of the donor fluorophore, and (b) receive, from the light sensor, a first measurement of the fluorescent light emitted from the acceptor fluorophore, (ii) during a second time period: (a) drive a second light source to generate light having a second illumination peak wavelength appropriate for direct excitation of the acceptor fluorophore, and (b) receive, from the light sensor, a second measurement of the fluorescent light emitted from the acceptor fluorophore, and (iii) calculate the concentration of the analyte based on the measurements.

Claims

1. Apparatus for detecting a concentration of an analyte in a subject, the apparatus comprising:

an implantable unit, which is configured to be implanted in a body of the subject, and comprises;

(a) fluorescent sensor molecules, each of which comprises (i) a binding site for the analyte, (ii) a donor fluorophore, and (iii) an acceptor fluorophore;

(b) a first implantable-unit light source that is configured to generate light having a first illumination peak wavelength appropriate for excitation of the donor fluorophore;

(c) a second implantable-unit light source that is configured to generate light having a second illumination peak wavelength appropriate for direct excitation of the acceptor fluorophore; and

(d) a third implantable-unit light source configured to emit light having a peak wavelength appropriate for effective penetration through tissue; and

an external system, which is physically separate and distinct from the implantable unit, and which comprises;

(a) an external reading unit, which comprises a light sensor, which is configured to sense fluorescent light emitted from the acceptor fluorophore; and

(b) one or more processors, which are configured to;

(i) during one or more first time periods;

(a) drive the first implantable-unit light source to generate the light having the first illumination peak wavelength, and (b) receive, from the light sensor, one or more first measurements of the fluorescent light emitted from the acceptor fluorophore, and

(ii) during one or more second time periods non-overlapping with the first time periods;

(a) drive the second implantable-unit light source to generate the light having the second illumination peak wavelength, and (b) receive, from the light sensor, one or more second measurements of the fluorescent light emitted from the acceptor fluorophore,

wherein the apparatus is configured to measure a temperature at the implantable unit,

wherein the implantable unit is configured to use the third implantable-unit light source to transmit an optical signal indicative of the measured temperature, wherein the strength of the optical signal emitted by the third implantable-unit light source does not vary based on a level of the fluorescent sensor molecules or a concentration of the analyte, and

wherein the one or more processors of the external system are configured to generate calibration information using the measured temperature, and calculate the concentration of the analyte in the subject based on the first and the second measurements and using the calibration information.

2. The apparatus according to claim 1, wherein the external reading unit is configured to drive the first implantable-unit light source and the second implantable-unit light source to generate the light having the first and the second illumination peak wavelengths, respectively, by transmitting electromagnetic radiation at first and second different frequencies, respectively.

3. The apparatus according to claim 1, wherein the implantable unit further comprises at least one sensor molecule chamber, in which the fluorescent sensor molecules are disposed.

4. The apparatus according to claim 1, wherein the fluorescent sensor molecules are fluorescent sensor proteins.

5. The apparatus according to claim 1, wherein the first illumination peak wavelength is greater than 300 nm and less than 550 nm.

6. The apparatus according to claim 5, wherein the first illumination peak wavelength is less than 525 nm.

7. The apparatus according to claim 6, wherein the first illumination peak wavelength is less than 500 nm.

8. The apparatus according to claim 1, wherein the second illumination peak wavelength is greater than 300 nm and less than 650 nm.

9. The apparatus according to claim 1, wherein the first illumination peak wavelength is greater than 300 nm and less than 550 nm, and the second illumination peak wavelength is greater than 300 nm and less than 650 nm.

10. The apparatus according to claim 1, wherein the acceptor fluorophore is configured to emit the fluorescent light having an emission peak wavelength that is between 100 and 500 nm greater than the first illumination peak wavelength.

11. The apparatus according to claim 1, wherein the analyte is glucose, and wherein the binding site is for the glucose.

12. The apparatus according to claim 1, wherein the implantable unit has a volume of no more than 250 mm³.

13. The apparatus according to claim 1, further comprising packaging, in which the implantable unit is stored before implantation, wherein an external surface of the implantable unit is sterile while stored in the packaging.

14. The apparatus according to claim 1,

wherein the implantable unit further comprises implant circuitry, which (a) is configured to generate a train of light pulses using the third implantable-unit light source, and (b) comprises at least one temperature-sensitive element,

wherein the implantable unit is configured such that the at least one temperature-sensitive element causes a change in time between successive light pulses as a function of temperature, and

wherein the external system is configured to identify the change in time by analyzing the received optical signal.

15. The apparatus according to claim 1,

wherein the implantable unit further comprises implant circuitry, and

wherein the one or more processors are configured to drive the first implantable-unit light source to generate the light having the first illumination peak wavelength by transmitting energy to the implant circuitry to activate the first implantable-unit light source to generate the light having the first illumination peak wavelength.

16. The apparatus according to claim 1, wherein the implantable unit is configured to drive the third implantable-unit light source to transmit the optical signal during one or more third time periods non-overlapping with the first and second time periods.

17. A method for detecting a concentration of an analyte in a subject, the method comprising:

implanting, in a body of the subject, an implantable unit, wherein the implantable unit is configured to measure a temperature at the implantable unit, and wherein the implantable unit comprises;

(a) fluorescent sensor molecules, each of which comprises (i) a binding site for the analyte, (ii) a donor fluorophore, and (iii) an acceptor fluorophore;

(b) a first implantable-unit light source that is configured to generate light having a first illumination peak wavelength appropriate for excitation of the donor fluorophore;

(c) a second implantable-unit light source that is configured to generate light having a second illumination peak wavelength appropriate for direct excitation of the acceptor fluorophore;

(d) a third implantable-unit light source configured to emit light having a peak wavelength appropriate for effective penetration through tissue, wherein the implantable unit is configured to use the third implantable-unit light source to transmit an optical signal indicative of the measured temperature, wherein the strength of the optical signal emitted by the third implantable-unit light source does not vary based on a level of the fluorescent sensor molecules or a concentration of the analyte; and

placing an external reading unit of an external system above skin of the subject, wherein the external system includes one or more processors, and wherein the external reading unit comprises a light sensor, which is configured to sense fluorescent light emitted from the acceptor fluorophore, wherein the external system is physically separate and distinct from the implantable unit, and when the external reading unit has been placed above the skin;

(a) during one or more first time periods;

(i) driving the first implantable-unit light source to generate the light having the first illumination peak wavelength, and (ii) receiving, from the light sensor, one or more first measurements of the fluorescent light emitted from the acceptor fluorophore,

(b) during one or more second time periods non-overlapping with the first time periods;

(i) driving the second implantable-unit light source to generate light having the second illumination peak wavelength appropriate for direct excitation of the acceptor fluorophore, and (ii) receiving, from the light sensor, one or more second measurements of the fluorescent light emitted from the acceptor fluorophore,

(c) during one or more third time periods non-overlapping with the first and second time periods;

(i) driving the third implantable-unit light source to generate the optical signal, and (ii) receiving the optical signal generated by the third implantable-unit light source,

(d) generating calibration information using the measured temperature, and

(e) calculating the concentration of the analyte in the subject based on the first and the second measurements and using the calibration information.

18. The method according to claim 17, wherein implanting the implantable unit comprises implanting the implantable unit that:

further comprises implant circuitry, which (a) is configured to generate a train of light pulses using the third implantable-unit light source, and (b) comprises at least one temperature-sensitive element,

is configured such that the at least one temperature-sensitive element causes a change in time between successive light pulses as a function of temperature, and wherein the external system is configured to identify the change in time by analyzing the received optical signal.