FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
First Claim
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1. A method of cleaving a FAD2 loci in a Brassica spp plant cell, the method comprising:
- introducing a nuclease comprising a DNA-binding domain and a cleavage domain or cleavage half-domain into the cell, wherein the DNA-binding domain binds to a target site within any of SEQ ID Nos;
17-26, 28-33 or 35-38 in the FAD2 loci and the nuclease cleaves the FAD2 loci.
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Abstract
A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.
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Citations
15 Claims
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1. A method of cleaving a FAD2 loci in a Brassica spp plant cell, the method comprising:
- introducing a nuclease comprising a DNA-binding domain and a cleavage domain or cleavage half-domain into the cell, wherein the DNA-binding domain binds to a target site within any of SEQ ID Nos;
17-26, 28-33 or 35-38 in the FAD2 loci and the nuclease cleaves the FAD2 loci. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
- introducing a nuclease comprising a DNA-binding domain and a cleavage domain or cleavage half-domain into the cell, wherein the DNA-binding domain binds to a target site within any of SEQ ID Nos;
Specification