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FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

  • US 10,577,616 B2
  • Filed: 04/02/2018
  • Issued: 03/03/2020
  • Est. Priority Date: 09/07/2012
  • Status: Active Grant
First Claim
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1. A method of cleaving a FAD2 loci in a Brassica spp plant cell, the method comprising:

  • introducing a nuclease comprising a DNA-binding domain and a cleavage domain or cleavage half-domain into the cell, wherein the DNA-binding domain binds to a target site within any of SEQ ID Nos;

    17-26, 28-33 or 35-38 in the FAD2 loci and the nuclease cleaves the FAD2 loci.

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