Biomedical patches with aligned fibers

• US 10,588,734 B2
• Filed: 08/14/2019
• Issued: 03/17/2020
• Est. Priority Date: 06/17/2010
• Status: Active Grant

• Current Assignee: Washington University In St Louis
• Original Assignee: Washington University In St Louis
• Inventors: MacEwan, Matthew R., Xie, Jingwei, Ray, Zack, Xia, Younan
• Primary Examiner(s): Stewart, Alvin J
• Application Number:
  US16/540,335
• Publication Number:
  US 20200000570A1
• Time in Patent Office: 216 Days
• CPC Class Codes: A61F 2/0063 Implantable repair or support meshes, e.g. hernia meshes
• CPC Class Codes: A61F 2/02 Prostheses implantable into the body closure means for urethra or rectum or for artificial body openings therefor A61F2/0004
• CPC Class Codes: A61F 2/105 Skin implants, e.g. artificial skin
• CPC Class Codes: A61L 15/22 containing macromolecular materials
• CPC Class Codes: A61L 15/42 Use of materials characterised by their function or physical properties liquid bandages A61L26/00
• CPC Class Codes: A61L 27/14 Macromolecular materials
• CPC Class Codes: A61L 27/50 Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: B29C 48/02 Small extruding apparatus, e.g. handheld, toy or laboratory extruders
• CPC Class Codes: B29C 48/05 Filamentary, e.g. strands
• CPC Class Codes: B29C 48/142 using force fields, e.g. gravity or electrical fields B29C48/9165 takes precedence
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: D01D 5/0076 characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid D01D5/0046 takes precedence
• CPC Class Codes: D01D 5/0092 characterised by the electrical field, e.g. combined with a magnetic fields, using biased or alternating fields
• CPC Class Codes: D04H 1/728 by electro-spinning electro-spinning methods and apparatus D01D5/0007
• CPC Class Codes: D04H 3/016 characterised by the fineness
• CPC Class Codes: D04H 3/073 Hollow cylinder shaped

Abstract

A three-dimensional electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate is provided. The scaffold includes a flexible deposited fiber network of varying density including a first and second set of set of electrospun fibers. The second set of electrospun fibers is coupled to the first. A first portion of the flexible deposited fiber network includes a higher density of fibers than a second portion of the flexible deposited fiber network, and the tensile strength of first portion is higher than that of the second portion. The scaffold is sufficiently flexible to facilitate application of scaffold to uneven surfaces of the tissue substrate, and enables movement of the scaffold by the tissue substrate. The first and second set of fibers are configured to degrade within three months after application, and each fiber of the deposited fiber network has a diameter of 1-1000 nanometers.

Claims

1. A three-dimensional electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate, the three-dimensional electrospun nanofiber scaffold comprising:

a flexible deposited electrospun fiber network of varying density, the deposited electrospun fiber network comprising;

a first set of deposited electrospun fibers comprising a first bioresorbable polymer, wherein the first bioresorbable polymer comprises polyglycolic acid; and

a second set of deposited electrospun fibers comprising a second bioresorbable polymer, the second set of deposited electrospun fibers coupled to the first set of deposited electrospun fibers,

wherein the first bioresorbable polymer comprises a different composition from the second bioresorbable polymer,

wherein a first portion of the flexible deposited electrospun fiber network comprises a higher density of fibers than a second portion of the flexible deposited electrospun fiber network, and wherein the first portion comprises a higher tensile strength than the second portion;

wherein the three-dimensional electrospun nanofiber scaffold comprises varying density to be sufficiently flexible to facilitate application of the three-dimensional electrospun nanofiber scaffold to uneven surfaces of the tissue substrate;

wherein the three-dimensional electrospun nanofiber scaffold comprises varying density to be sufficiently flexible to enable movement of the three-dimensional electrospun nanofiber scaffold by the tissue substrate,

wherein the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are configured to degrade within three months after application to the tissue substrate, and

wherein each fiber of the deposited electrospun fiber network comprises a diameter of 1-1000 nanometers.

2. The three-dimensional electrospun nanofiber scaffold of claim 1, wherein the second bioresorbable polymer comprises caprolactone.

3. The three-dimensional electrospun nanofiber scaffold of claim 1, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are radially aligned.

4. The three-dimensional electrospun nanofiber scaffold of claim 1, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are non-radially aligned.

5. The three-dimensional electrospun nanofiber scaffold of claim 1, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are randomly oriented.

6. The three-dimensional electrospun nanofiber scaffold of claim 1, wherein the deposited electrospun fiber network comprises a single layer.

7. The three-dimensional electrospun nanofiber scaffold of claim 1, wherein the deposited electrospun fiber network comprises multiple layers.

8. A three-dimensional electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate, the three-dimensional electrospun nanofiber scaffold comprising:

a flexible deposited electrospun fiber network of varying density, the deposited electrospun fiber network comprising;

a first set of deposited electrospun fibers comprising a first bioresorbable polymer, wherein the first bioresorbable polymer comprises glycolic acid; and

a second set of deposited electrospun fibers comprising a second bioresorbable polymer, the second set of deposited electrospun fibers coupled to the first set of deposited electrospun fibers,

wherein the first bioresorbable polymer comprises a different composition from the second bioresorbable polymer,

wherein at least a first portion of the flexible deposited electrospun fiber network comprises a higher density of fibers than a second portion of the flexible deposited electrospun fiber network, and wherein the first portion comprises a higher tensile strength than the second portion;

wherein the three-dimensional electrospun nanofiber scaffold comprises varying density to be conformable to the defect in the tissue substrate,

wherein the three-dimensional electrospun nanofiber scaffold comprises varying density to be sufficiently flexible to enable movement of the three-dimensional electrospun nanofiber scaffold by the tissue substrate,

wherein the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are configured to separate from each other within three months after application to the tissue substrate, and

wherein each fiber of the deposited electrospun fiber network comprises a diameter of 1-1000 nanometers.

9. The three-dimensional electrospun nanofiber scaffold of claim 8, wherein the second bioresorbable polymer comprises caprolactone.

10. The three-dimensional electrospun nanofiber scaffold of claim 8, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are radially aligned.

11. The three-dimensional electrospun nanofiber scaffold of claim 8, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are non-radially aligned.

12. The three-dimensional electrospun nanofiber scaffold of claim 8, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are randomly oriented.

13. The three-dimensional electrospun nanofiber scaffold of claim 8, wherein the deposited electrospun fiber network comprises a single layer.

14. The three-dimensional electrospun nanofiber scaffold of claim 8, wherein the deposited electrospun fiber network comprises multiple layers.

15. A monolayer electrospun nanofiber patch for use in repairing a defect in a tissue substrate, the monolayer electrospun nanofiber patch comprising:

a flexible deposited electrospun fiber network of varying density, the deposited electrospun fiber network comprising;

a first set of deposited electrospun fibers comprising a first bioresorbable polymer, wherein the first bioresorbable polymer comprises glycolic acid; and

a second set of deposited electrospun fibers comprising a second bioresorbable polymer, the second set of deposited electrospun fibers coupled to the first set of deposited electrospun fibers,

wherein the first bioresorbable polymer comprises a different composition from the second bioresorbable polymer,

wherein an inner portion of the monolayer electrospun nanofiber patch comprises a different fiber density than an outer portion of the monolayer electrospun nanofiber patch;

wherein the monolayer electrospun nanofiber patch comprises varying density to be conformable to the defect in the tissue substrate,

wherein the monolayer electrospun nanofiber patch comprises varying density to be sufficiently flexible to enable movement of the monolayer electrospun nanofiber patch by the tissue substrate,

wherein the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are configured to degrade within three months after application to the tissue substrate, and

wherein each fiber of the deposited electrospun fiber network comprises a diameter of 1-1000 nanometers.

16. The monolayer electrospun nanofiber patch of claim 15, wherein the second bioresorbable polymer comprises caprolactone.

17. The monolayer electrospun nanofiber patch of claim 15, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are radially aligned.

18. The monolayer electrospun nanofiber patch of claim 15, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are non-radially aligned.

19. The monolayer electrospun nanofiber patch of claim 15, wherein at least one of the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are randomly oriented.

20. The monolayer electrospun nanofiber patch of claim 15, wherein the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are non-radially aligned.