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Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases

  • US 10,588,983 B2
  • Filed: 02/23/2018
  • Issued: 03/17/2020
  • Est. Priority Date: 02/23/2005
  • Status: Active Grant
First Claim
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1. A method for modulating the immune system in a subject, comprising subcutaneously administering to a subject immune globulin (IG) and a soluble hyaluronidase to enhance the immune system of the subject, wherein:

  • the soluble hyaluronidase is a soluble neutral active human hyaluronidase glycoprotein (sHASEGP);

    the hyaluronidase is active at neutral pH and contains at least one sugar moiety that is covalently attached to an asparagine (N) residue of the hyaluronidase polypeptide;

    the hyaluronidase polypeptide does not comprise the complete sequence of amino acids set forth in SEQ ID NO;

    1;

    and the hyaluronidase polypeptide consists of;

    (i) a contiguous sequence of amino acid residues contained within SEQ ID NO;

    1 that includes at least amino acids 36-464 of SEQ ID NO;

    1, wherein the polypeptide is truncated within the C-terminus of SEQ ID NO;

    1 at a C-terminal amino acid residue selected from among 477, 478, 479, 480, 481, 482, 483 and 494 of SEQ ID NO;

    1;

    or(ii) a sequence of amino acid residues that has at least 95% amino acid sequence identity with a sequence of amino acids set forth in (i).

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