Reagent storage in microfluidic systems and related articles and methods

• US 10,589,265 B2
• Filed: 12/13/2017
• Issued: 03/17/2020
• Est. Priority Date: 12/18/2008
• Status: Active Grant

• Current Assignee: Opko Diagnostics, LLC (Opko Health, Inc.)
• Original Assignee: Opko Diagnostics, LLC (Opko Health, Inc.)
• Inventors: Taylor, Jason, Linder, Vincent
• Primary Examiner(s): White, Dennis
• Assistant Examiner(s): Kilpatrick, Bryan
• Application Number:
  US15/840,159
• Publication Number:
  US 20180099272A1
• Time in Patent Office: 825 Days
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2200/142 Preventing evaporation
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 2300/161 Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/527 for a plurality of reagents
• CPC Class Codes: B01L 3/561 Tubes; Conduits in general F16L

Abstract

Fluidic devices and methods including those that provide storage and/or facilitate fluid handling of reagents are provided. Fluidic devices described herein may include channel segments positioned on two sides of an article, optionally connected by an intervening channel passing through the article. The channel segments may be used to store reagents in the device prior to first use by an end user. The stored reagents may include fluid plugs positioned in linear order so that during use, as fluids flow to a reaction site, they are delivered in a predetermined sequence. The specific geometries of the channel segments and the positions of the channel segments within the fluidic devices described herein may allow fluid reagents to be stored for extended periods of time without mixing, even during routine handling of the devices such as during shipping of the devices, and when the devices are subjected to physical shock or vibration.

Claims

1. A fluidic device comprising:

an article comprising a top surface, a bottom surface, a thickness, a first microfluidic channel segment formed in the top surface of the article, and a second microfluidic channel segment formed in the bottom surface of the article;

an intervening channel passing through the thickness of the article and connecting the first microfluidic segment formed in the top surface of the article and the second microfluidic channel segment formed in the bottom surface of the article;

a first cover positioned over the first microfluidic channel segment on the top surface of the article;

a second cover positioned over the second microfluidic channel segment on the bottom surface of the article;

an inlet in fluid communication with the first and second microfluidic channel segments;

an outlet in fluid communication with the first and second microfluidic channel segments;

a third cover covering the inlet; and

a fourth cover covering the outlet.

2. The fluidic device of claim 1, comprising a plurality of the first and second microfluidic channel segments.

3. The fluidic device of claim 1, wherein the article is a single, integral piece of material without joined layers.

4. The fluidic device of claim 1, wherein two planes tangent to two points on a perimeter of a cross section of the first channel segment intersect at an angle of less than or equal to 45°

, wherein the two points are on adjacent walls of the first channel segment, at least one of the walls being a portion of a cover enclosing at least a portion of the first channel segment.

5. The fluidic device of claim 1, wherein the fluidic device comprises a channel that is not in fluid communication with the first and second microfluidic channel segments, and wherein a stored, dry reagent is a reagent immobilized on at least a portion of the channel that is not in fluid communication with the first and second microfluidic channel segments.

6. The fluidic device of claim 1, wherein the intervening channel has a cross-sectional shape different than the cross-sectional shapes of the first and/or second microfluidic channel segments.

7. The fluidic device of claim 1, wherein the first microfluidic channel segment and/or the second microfluidic channel segment has at least one angle between adjacent walls of the first and/or second microfluidic channel segment of greater than or equal to 3°

and less than 90°

.

8. The fluidic device of claim 1, wherein the first microfluidic channel segment and the first cover mate such that a cross-section of the first microfluidic channel segment, when mated with the first cover, includes a first portion adjacent the first cover that is convex and a second portion continuous with the first portion that is linear or concave.

9. The fluidic device of claim 8, wherein the convex portion comprises a radius of curvature of greater than or equal to at least 5 microns.

10. The fluidic device of claim 9, wherein the convex portion is continuous along the length of the first microfluidic channel segment.

11. The fluidic device of claim 1, wherein no more than 5% of the perimeter of a cross section of the first microfluidic channel segment is perpendicular to the first surface and/or wherein no more than 5% of the perimeter of a cross section of the second microfluidic channel segment is perpendicular to the second surface.

12. The fluidic device of claim 1, wherein the intervening channel has cross-sectional dimensions within 50% of the smallest width of each of the first and second microfluidic channel segments.

13. The fluidic device of claim 1, wherein the intervening channel has a ratio of length to largest width of less than 3.

14. The fluidic device of claim 1, wherein the first and/or second microfluidic channel segments have a width to depth ratio of greater than or equal to 1.

15. The fluidic device of claim 1, wherein the first and/or second microfluidic channel segments have a cross-sectional dimension of less than 600 microns.

16. The fluidic device of claim 1, wherein the article has a thickness of less than 3 mm.

17. The fluidic device of claim 1, wherein the intervening channel has a cross sectional shape that resembles a circle.

18. The fluidic device of claim 1, wherein the intervening channel has a cross-sectional dimension that varies along at least a portion of the thickness of the article.

19. The fluidic device of claim 1, wherein the article comprises multiple substrate layers that are mated to one another.

20. The fluidic device of claim 1, wherein the length of the first microfluidic channel segment is at least 10 times greater than the length of the second microfluidic channel segment.

21. The fluidic device of claim 1, wherein the first cover is the same as the third cover and/or the fourth cover.

22. The fluidic device of claim 1, wherein the second cover is the same as the third cover and/or the fourth cover.